HAYASHI Lab.
Just another hayashi Lab. site
  • Japanese
  • English

Department of Applied Chemistry, Graduate School of Engineering, Osaka University

  • Home
  • Research
    • Research
    • collaborations
  • Prof. HAYASHI
  • Group Members
  • Publications
    • Original Papers and Reviews
    • Cover
  • Resources
  • Pictures
    • Event
    • Group Picture
    • Lab. Tour
  • Guests
  • Contacts

Koji OOHORA Ph.D.

Koji OOHORA, Ph.D.Associate Professor
Affiliation

Department of Applied Chemistry,
Graduate School of Engineering,
Osaka University

Address

Building/Room #C4-623
2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

Contact

E-mail: oohora ”at” chem.eng.osaka-u.ac.jp ( ”at” = @)
Phone: +81-(0)6-6879-7929     Fax:  +81-(0)6-6879-7930

Career

Education

B. Eng., Osaka University, March 2007
M. Eng., Osaka University, March 2009
Ph.D. in Eng., Osaka University, September 2011 (supervisor: Prof. Takashi Hayashi)

Professional Career

JSPS (Japan Society of the Promotion of Science) Fellow (DC1) 2009–2011
Assistant Professor, Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2011–2015
Assistant Professor, Frontier Research Base for Global Young Researchers, Graduate School of Engineering, Osaka University, 2015–2020
Project Leader on Precursory Research for Embryonic Science and Technology (PRESTO), in Japan Science and Technology Agency, 2015– 2019
Associate Professor, Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2020–Current

Activities

Memberships

Chemical Society of Japan (CSJ),
Japan Society of Coordination Chemistry (JSCC),
Society of Biological Inorganic Chemistry (SBIC),
Society of Porphyrins and Phthalocyanines (SPP)

Awards
2008         The 4th Asian Biological Inorganic Chemistry Conference      
                    Young Scientist Oral Presentation Award      
2011         15th International Conference on Biological Inorganic Chemistry      
                    SBIC Student Travel Grants      
2013          Inoue Research Award for Young Scientists      
2013          CSJ Presentation Award 2013      
2014          Symposium on Bio-related Chemistry Oral Presentation Award 2014      
2015          Chancellor’s Incentive Award from Osaka University, 2015      
2019          PCCP Prize 2019      
2019          CSJ (Chemical Society of Japan) Presentation Award 2019      
2019         Early Career Award in 9th International Symposium on Bioorganometallic Chemistry      
2021         The CSJ Award for Young Chemists       
2021          JSCC (Japan Society of Coordination Chemistry) Research Encouragement Award      

Research Interests

Research Interest

Biological Inorganic Chemistry, Supramolecular Chemistry, Protein Engineering, Nano-biotechnology

Selected Papers

Papers

 Google Scholar: Koji Oohora

1. Supramolecular Hemoprotein Linear Assembly by Successive interprotein Heme–Heme Pocket Interactions
Hiroaki Kitagishi, Koji Oohora, Hiroyasu Yamaguchi, Hideki Sato, Takashi Matsuo, Akira Harada, Takashi Hayashi* 
 
J. Am. Chem. Soc., 129, 10326–10327 (2007). 


2. Self-Assembly of One- and Two-Dimensional Hemoprotein Systems by Polymerization through Heme–Heme Pocket Interactions
Hiroaki Kitagishi, Yasuaki Kakikura, Hiroyasu Yamaguchi, Koji Oohora, Akira Harada, Takashi Hayashi* 

Angew. Chem., Int. Ed., 48, 1271–1274 (2009). Issue Picture

3. Thermodynamically Controlled Supramolecular Polymerization of Cytochrome b562
Hiroaki Kitagishi, Koji Oohora, Takashi Hayashi* 

Biopolymers, 3, 194–200 (2009). Front Cover 

4. A Chemically-controlled Supramolecular Protein Polymer Formed by a Myoglobin-based Self-assembly System
Koji Oohora, Akira Onoda, Hiroaki Kitagishi, Hiroyasu Yamaguchi, Akira Harada, Takashi Hayashi*
Chem. Sci., 2, 1033–1038, (2011). Backside Cover.

5. Chemically Programmed Supramolecular Assembly of Hemoprotein and Streptavidin with Alternating Alignment
Koji Oohora, Sabina Burazerovic, Akira Onoda, Yvonne M. Wilson, Thomas R. Ward* Takashi Hayashi*
Angew. Chem., Int. Ed., 51, 3818–3821 (2012). Front Cover. 

6. Fibrous Supramolecular Assemblies Connected with Synthetic Heme Dimer and Apohemoprotein Dimer
Akira Onoda, Akinori Takahashi, Koji Oohora, Yoshitaka Onuma, Takashi Hayashi*
Chem. Biodivers., 9, 1684–1692 (2012). 

7. Supramolecular Assembling Systems Formed by Heme–Heme Pocket Interactions in Hemoproteins
Koji Oohora, Akira Onoda, Takashi Hayashi*
Chem. Commun., 48, 11714–11726 (2012). Feature Article.

8. Photoinduced Electron Transfer of ZnS−AgInS2 Solid-Solution Semiconductor Nanoparticles: Emission Quenching and Photocatalytic Reactions Controlled by Electrostatic Forces
Taro Uematsu, Akihisa Doko, Tsukasa Torimoto, Koji Oohora, Takashi Hayashi, Susumu Kuwabata*
J. Phys. Chem. C, 117, 15667–15676 (2013).

9. C(sp3)–H Bond Hydroxylation Catalyzed by Myoglobin Reconstituted with Manganese Porphycene
Koji Oohora, Yushi Kihira, Eiichi Mizohata, Tsuyoshi Inoue, Takashi Hayashi* 
J. Am. Chem. Soc., 135, 17282–17285 (2013). 

10. “Hemoprotein-based Supramolecular Assembling Systems”
Koji Oohora, and Takashi Hayashi*

 Curr. Opin. Chem. Biol., 19, 154–161 (2014).   
11. “Co(II)/Co(I) Reduction-induced Axial Histidine-flipping in Myoglobin Reconstituted with a Cobalt Tetradehydrocorrin as a Methionine Synthase Model”
Takashi Hayashi,* Yoshitsugu Morita, Eiichi Mizohata, Koji Oohora, Jun Ohbayashi, Tsuyoshi Inoue, and Yoshio Hisaeda

 Chem. Commun., 50, 12560–12563 (2014). Open Access.
12. “meso-Dibenzoporphycene has a Large Bathochromic Shift and a Porphycene Framework with an Unusual Cis Tautomeric Form”
Koji Oohora, Ayumu Ogawa, Tamaki Fukuda, Akira Onoda, Jun-ya Hasegawa,* and Takashi Hayashi* 

Angew. Chem., Int. Ed., 54, 6227–6230 (2015). 
13. “Energy Migration within Hexameric Hemoprotein Reconstituted with Zn Porphyrinoid Molecules”
Koji Oohora, Tsuyoshi Mashima, Kei Ohkubo, Shunichi Fukuzumi, and Takashi Hayashi* 

Chem. Commun., 51, 1138–1140 (2015). Open Access.
14. “Intraprotein Transmethylation via a CH3–Co(III) Species in Myoglobin Reconstituted with a Cobalt Corrinoid Complex”
Yoshitsugu Morita, Koji Oohora, Akiyoshi Sawada, Kazuki Doitomi, Jun Ohbayashi, Takashi Kamachi, Kazunari Yoshizawa, Yoshio Hisaeda, and Takashi Hayashi* 

Dalton Trans., 45, 3277–3284 (2016). Open Access. Front Cover.
15. “Crystal Structures and Coordination Behavior of Aqua- and Cyano-Co(III) Tetradehydrocorrins in the Heme Pocket of Myoglobin”
Yoshitsugu Morita, Koji Oohora, Eiichi Mizohata, Akiyoshi Sawada, Takashi Kamachi, Kazunari Yoshizawa, Tsuyoshi Inoue, and Takashi Hayashi*  

Inorg. Chem., 55, 1287–1295 (2016).
16. “Oxygen-binding Protein Fiber and Microgel: Supramolecular Myoglobin-Poly(acrylate) Conjugates”
Toshikazu Ono, Yasushi Hisaoka, Akira Onoda,* Koji Oohora, and Takashi Hayashi*  

Chem. Asian J., 11, 1036-1042 (2016).
17. “Redox Potentials of Cobalt Corrinoids with Axial Ligands Correlate with Heterolytic Co–C Bond Dissociation Energies”
Yoshitsugu Morita, Koji Oohora, Akiyoshi Sawada, Takashi Kamachi, Kazunari Yoshizawa, and  Takashi Hayashi*  

Inorg. Chem., 56, 1950–1955 (2017).
18. “Cobalt tetradehydrocorrins coordinated by imidazolate-like histidine in the heme pocket of horseradish peroxidase”
Koji Oohora, Ning Tang, Yoshitsugu Morita, and  Takashi Hayashi*  

J. Biol. Inorg. Chem., 22, 695–703 (2017) .
19. “Iron-Strapped Porphyrins with Carboxylic Acid Groups Hanging over the Coordination Site: Synthesis, X-ray Characterization, and Dioxygen Binding”
Bernard Boitrel*, Ismail Hijazi, Thierry Roisnel, Koji Oohora, and Takashi Hayashi*

Inorg. Chem.,  56, 7373–7383 (2017).
20. “A supramolecular assembly based on an engineered hemoprotein exhibiting a thermal stimulus-driven conversion to a new distinct supramolecular structure”
Koji Oohora, Yoshitaka Onuma, Yuta Tanaka, Akira Onoda, and Takashi Hayashi*

Chem. Commun., 53, 6879–6882  (2017).
21. “CuAAC in a Distal Pocket: Metal Active-Template Synthesis of Strapped-Porphyrin [2]Rotaxanes”
Yuta Miyazaki, Christophe Kahlfuss, Ayumu Ogawa, Takashi Matsumoto, Jennifer A. Wytko, Koji Oohora, Takashi Hayashi,* and Jean Weiss*

Chem. Eur. J., 23, 13579–13582  (2017), Front Cover.
22. “Catalytic Cyclopropanation by Myoglobin Reconstituted with Iron Porphycene: Acceleration of Catalysis due to Rapid Formation of the Carbene Species”
Koji Oohora, Hiroyuki Meichin, Liming Zhao, Matthew W, Wolf, Akira Nakayama, Jun-ya Hasegawa,* Nicolai Lehnert* and Takashi Hayashi*

J. Am. Chem. Soc., 139, 17265–17268  (2017).
23. “Manganese(V) Porphycene Complex Responsible for Inert C–H Bond Hydroxylation in a Myoglobin Matrix”
Koji Oohora*, Hiroyuki Meichin, Yushi Kihira, Hiroshi Sugimoto, Yoshitsugu Shiro, and Takashi Hayashi*

J. Am. Chem. Soc.,139, 18460–18463 (2017).
24. “Substitution of an amino acid residue axially coordinating to the heme molecule in hexameric tyrosine-coordinated hemoprotein to enhance peroxidase activity”
Tsuyoshi Mashima, Koji Oohora,* and Takashi Hayashi*

J. Porphyrins Phthalocyanines, 21, 824–831 (2017).
25. “Substitution of an amino acid residue axially coordinating to the heme molecule in hexameric tyrosine-coordinated hemoprotein to enhance peroxidase activity”
Shumpei Hanai, Hirofumi Tsujino, Taku Yamashita,* Ryo Torii, Hitomi Sawai, Yoshitsugu Shiro, Koji Oohora, Takashi Hayashi and Tadayuki Uno*

J. Inorg. Biochem.,179, 1–9 (2018).
26. “Successive Energy Transfer within Multiple Photosensitizers Assembled in a Hexameric Hemoprotein Scaffold”
Tsuyoshi Mashima,  Koji Oohora* and Takashi Hayashi*

Phys. Chem. Chem. Phys., 20, 3200–3209  (2018).
27. “Preparation and characterization of myoglobin reconstituted with Fe(II) oxaporphyrin: The monoanionic macrocycle provides unique cyanide binding behavior for the ferrous species”
Hiroyuki, Meichin, Koji Oohora, and Takashi Hayashi*

Inorg. Chim. Acta, 472, 184–191 (2018).
28. “Supramolecular Hemoprotein Assembly with a Periodic Structure Showing Heme–Heme Exciton Coupling”
Koji Oohora,* Nishiki Fujimaki, Ryota Kajihara, Hiroki Watanabe, Takayuki Uchihashi,* and Takashi Hayashi*

J. Am. Chem. Soc., 140, 10145–10148 (2018).
29. “Synthesis and Characterization of meso-Substituted Cobalt Tetradehydrocorrin and Evaluation of Its Electrocatalytic Behavior Toward CO2 Reduction and H2 Evolution”
Ayumu Ogawa, Koji Oohora* and Takashi Hayashi*

Inorg. Chem., 57, 14644–14652 (2018).
30. “Electrochemical CO2 reduction by a cobalt bipyricorrole complex: decrease of an overpotential value derived from monoanionic ligand character of the porphyrinoid species”
Ayumu Ogawa, Koji Oohora,* Wenting Gu and Takashi Hayashi*

Chem. Commun., 55, 493–496 (2019).
31. “A ring-shaped hemoprotein trimer thermodynamically controlled by the supramolecular heme–heme pocket interaction”
Koji Oohora,* Ryota Kajihara, Nishiki Fujimaki, Takayuki Uchihashi* and Takashi Hayashi*

Chem. Commun., 55, 1544–1547 (2019), Front Cover.
32. “Photoinduced electron transfer within supramolecular hemoprotein co-assemblies and heterodimers containing Fe and Zn porphyrins”
Ryota Kajihara, Koji Oohora,* and Takashi Hayashi*

J. Inorg. Biochem., 193, 42–51 (2019).
33. “Arginine Residues Provide a Multivalent Effect for Cellular Uptake of a Hemoprotein Assembly”
Koji Oohora,* Ryota Kajihara, Misa Jiromaru, Hiroaki Kitagishi* and Takashi Hayashi*

Chem. Lett., 48, 295–298 (2019).
34. “Light Triggers Molecular Shuttling in Rotaxanes: Control over Proximity and Charge Recombination”
Maximilian Wolf,a Ayumu Ogawa, Mareike Bechtold, Maxime Vonesch, Jennifer A. Wytko, Koji Oohora, Stéphane Campidelli, Takashi Hayashi,* Dirk M. Guldi,* and Jean Weiss*

Chem. Sci., 10, 3846–3853 (2019).
35. “Hemoproteins Reconstituted with Artificial Metal Complexes as Biohybrid Catalysts”
Koji Oohora, Akira Onoda and Takashi Hayashi*

Acc. Chem. Res., 52, 945–954 (2019).
36. “Myoglobin Reconstituted with Ni Tetradehydrocorrin as a Methane Generating Model of Methyl‐coenzyme M Reductase”
Koji Oohora,* Yuta Miyazaki and Takashi Hayashi*

Angew. Chem. Int. Ed., 58, 13813–13817 (2019)
37. “Supramolecular dimerization of a hexameric hemoprotein via multiple pyrene–pyrene interactions”
Koji Oohora,*  Shota Hirayama, Tsuyoshi Mashima and Takashi Hayashi*

J. Porphyrins Phthalocyanines, 24, 259–267 (2020).
38. “Methane generation via intraprotein C–S bond cleavage in cytochrome b562 reconstituted with nickel didehydrocorrin”
Yuta Miyazaki, Koji Oohora* and Takashi Hayashi*

J. Organomet. Chem., 901, 120945 (2020).
39. “Construction of a Hexameric Hemoprotein Sheet and Direct Observation of Dynamic Process of its Formation”
Koji Oohora,* Shota Hirayama, Takayuki Uchihashi* and Takashi Hayashi*

Chem. Lett., 49, 186-190 (2020).
40. “A Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-harvesting System”
Shota Hirayama, Koji Oohora,* Takayuki Uchiahashi* and Takashi Hayashi*

J. Am. Chem. Soc., 142. 1822–1831 (2020).
 
41. "Methane Generation and Reductive Debromination of Benzylic Position by Reconstituted Myoglobin Containing Nickel Tetradehydrocorrin as a Model of Methyl-coenzyme M Reductase”
Yuta Miyazaki, Koji Oohora,* and Takashi Hayashi*
 Inorg. Chem., 59, 11995-12004 (2020).
 
42. “Effect of Molecule–Substrate Interactions on the Adsorption of meso-Dibenzoporphycene Tautomers Studied by Scanning Probe Microscopy and First-Principles Calculations”
Tomoko K. Shimizu, Carlos Romero-Muñiz, Oleksandr Stetsovych, Jaime Carracedo-Cosme, Michael Ellner, Pablo Pou, Koji Oohora, Takashi Hayashi, Ruben Perez* and Oscar Custance* 
 J. Phys. Chem. C, 124, 26759-26768 (2020).
 
43. "Myoglobins engineered with artificial cofactors serve as artificial metalloenzymes and models of natural enzymes”
 Koji Oohora* and Takashi Hayashi*
 Dalton Trans.,  50, 1940-1949 (2021) Front Cover.
 
44. “A Supramolecular Assembly of Hemoproteins Formed in a Star-Shaped Structure via Heme–Heme Pocket Interactions”
Julian Wong Soon, Koji Oohora,* Shota Hirayama, and Takashi Hayashi*

Int. J. Mol. Sci., 22, 1012 (2021) Open Access.
45. “Dynamic Protease Activation on a Multimeric Synthetic Protein Scaffold via Adaptable DNA-Based Recruitment Domains”
Tsuyoshi Mashima, Bas J. H. M. Rosier, Koji Oohora, Tom F. A. de Greef, Takashi Hayashi, and Luc Brunsveld*
Angew. Chem. Int. Ed., 60, 11262–11266 (2021). Open Access. 
 
46. "Functional Myoglobin Model Composed of a Strapped Porphyrin/Cyclodextrin Supramolecular Complex with an Overhanging COOH That Increases O2/CO Binding Selectivity in Aqueous Solution"
Qiyue Mao, Pradip K. Das, Stéphane Le Gac, Bernard Boitrel,* Vincent Dorcet, Koji Oohora, Takashi Hayashi, and Hiroaki Kitagishi*
Inorg. Chem., 60, 12392–12404 (2021).
Books

1. Koji Oohora, Takashi Hayashi, “Incorporation of modified and artificial cofactors into naturally occurring protein scaffolds” In Methods in Molecular Biology, pp. 251–263,2014. 

2.Koji Oohora, Takashi Hayashi "Artificial Hemoprotein Assemblies in Development of Nanobiomaterials" In Nioinspired Chemistry From Enzymes to Synthetic Models, M. Réglier, Ed., World Scientific, 71–88 (2019). 

3. Koji Oohora, Takashi Hayashi "Myoglobin Derivatives Reconstituted with Modified Metal Porphyrinoids, as a Structural and Functional Models of the Cytochrome P450 Enzymes" In Dioxygene-dependent Heme Enzymes, M. Ikeda-Saito, E. Raven, Eds., RSC, 63–78 (2019).

Invited Lecture

1. Koji Oohora, “C–H Bond Hydroxylation Catalyzed by Manganese Porphycene in a Myoglobin Matrix”
Characterization and Reactivity of Metal-oxo Species Generated by a Metalloenzyme Model, S4-4, Sep. 2014, Chuo University, Tokyo

2. Koji Oohora "Preparation of hemoprotein-based supramolecular assemblies and artificial enzyme" 28th Summer School on Biological Inorganic Chemistry （Shizuoka, Japan）, 2015, Aug. 28th Invited Lecture

3. Koji Oohora "Design and engineering of myoglobin containing a manganese porphycene toward enantioselective hydroxylase" The 53th Annual Meeting of the Biophysical Society of Japan (Ishikawa, Japan), 2015, Sep. 14th Invited Lecture

4. Koji Oohora, Yasunobu Tanaka, Ayumu Ogawa, Takashi Hayashi "Synthesis, structure and physicochemical properties of meso-dibenzoporphycene" Theory, Measurement and Creation of Porphyrinoid Compounds as Soft Molecular Systems (Ibaraki, Japan)、2015, Oct. 1st Invited Lecture

5. Koji Oohora, Takashi Hayashi "H2O2-dependent hydroxylation of C–H bond by myoglobin reconstituted with a manganese porphycene complex" PACIFICHEM 2015 （Hawaii, USA）, 2015, Dec. 17th, Invited Lecture

6. Koji Oohora, Ayumu Ogawa, Yasunobu Tanaka, Jun-ya Hasegawa, Takashi Hayashi, “Dibenzoporphycene with Annulated Benzene Rings at Two Ethylene Bridges of the Porphycene Framework” Ninth International Conference on Porphyrins and Phthalocyanines （Nanjing, Japan）, 2016, Jul. 7th, Invited Lecture

7. Koji Oohora、"Modification of Hemoprotein: Construction of Supramolecular Assembly and Artificial Enzyme " Seminar on Function and Physical Property（Chiba, Japan）, 2016, Jul. 21st Invited Lecture 

8. Koji Oohora "Hemoprotein engineered with unnatural cofactors toward artificial metalloenzymes" The 97th Chemical Society of Japan Annual Meeting: Asian International Symposium -Coordination Chemistry, Organometallic Chemistry-, (Kanagawa, Japan) 2017, Mar. 17th Invited Lecture

9. Koji Oohora "Construction of Hemoprotein-based Artificial Light Harvesting System" 38th Summer School of the Japanese Photochemistry Association, （Fukuoka, Japan） 2017, Jun. 16th, Invited Lecture

10. Koji Oohora "Chemical Modification of Hemoprotein toward Biomaterials and Artificial Enzyme" Summer School in Young Coordination Chemist’s Association Japan 2017 (Aichi, Japan) 2017, Jul. 31st, Invited Lecture

11. Koji Oohora "Creation of Artificial Enzymes and Enzyme Models by Chemical Modification of Hemoprotein " ITbM/IGER Chemistry Workshop 2017 (Nagoya, Japan) 2017, Nov. 6th, Invited Lecture

12. Koji Oohora, Takashi Hayashi "An Array of Metal Porphyrinoid Chromophores Using Hemoprotein Assembly as a Scaffold" SNCPP18, （Ritsumeikan Univ., Shiga）2018. Jun.16th, Invited Lecture

13. Koji Oohora "Construction and photochemical properties of supramolecular assembly of hemoprotein" SUPRAMOLECULAR CHEMISTRY OF NITROGEN LIGANDS　（Univ. Strasbourg, France） 2018, Jun. 29th, Invited Lecture

14. Koji Oohora, Tang Ning, Yoshitsugu Morita, Takashi Hayashi, "Hemoproteins reconstituted with synthetic cobalt corrinoid toward models of vitamin B12-dependent enzymes" Tenth International Conference on Porphyrins and Phthalocyanines, (Westin Grand in Munich, Germany) 2018, Jul. 3rd, Invited Lecture

15. Koji Oohora,“Hemoproteins reconstituted with metal corrinoids as functional models of natural enzymes related to bioorganometallic species” 9th International Symposium on Bioorganometallic Chemistry (Univ. York, United Kingdom) 2019, Jul. 31st. Keynote Lecture

PAGE TOP
　
  • Copyright(c) 2015- Hayashi Lab, Osaka Univ. All Rights Reserved.