Department of Applied Chemistry, Graduate School of Engineering, Osaka University
Takashi HAYASHIProfessor of Chemistry
Department of Applied Chemistry,
Graduate School of Engineering,
2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
E-mail: thayashi ”at” chem.eng.osaka-u.ac.jp ( ”at” = @)
Phone: +81-(0)6-6879-7928 Fax: +81-(0)6-6879-7930
B. Eng., Kyoto University, March 1985
M. Eng., Kyoto University, March 1987(supervisor: Prof. Iwao Tabushi)
Ph.D. in Eng., Kyoto University, March 1990(supervior: Prof. Yoshihiko Ito)
- Professional Career
Assistant Professor, Faculty of Engineering, Kyoto University, 1990–1997
Visiting Scientist, The Scripps Research Institute, 1995–1996
Associate Professor, Faculty of Engineering, Kyushu University, 1997–2005
Project Leader on Precursory Research for Embryonic Science and Technology (PREST)
in Japan Science and Technology Corporation (JST), 2000–2003
Professor, Faculty of Engineering, Osaka University, 2005–present
Visiting Professor, Institute of Molecular Science, 2005–2007
Visiting Professor, University of Strasbourg, 2010.
Chemical Society of Japan (CSJ),
American Chemical Society (ACS),
Japan Society of Coordination Chemistry (JSCC),
Society of Biological Inorganic Chemistry (SBIC),
Society of Porphyrins and Phthalocyanines (SPP),
Society of Synthetic Organic Chemistry of Japan,
Society of Polymer Science of Japan,
Catalysis Society of Japan,
The Biophysical Society of Japan.
- Council Member
Division of the Biofunctional Chemistry and Biotechnology, CSJ, 2006-2010
Japan Society of Coordination Chemistry, JSCC, 2009-2011
- Editorial Boards
J. Porphyrins Phthalocyanines,
J. Inorg. Biochem.
Bulletin of Chemical Society of Japan (BCSJ) Associate Editor, 2005–2011
Kaneka Corporation Award in Synthetic Organic Chemistry (1992)
Inoue Research Award for Young Scientists (1992)
The Progress Award on Synthetic Organic Chemistry, Japan (2000)
1st Young Investigator Award in Porphyrin Chemistry (2000)
2001 Kyushu University President Award (2002)
7th Research Promoted Award on Enzyme Application supported by Amano Enzyme Inc. (2006)
BCSJ Award (2008)
The 27th Chemical Society of Japan Award for Creative Work (2010)
Osaka University Achievement Awards in Research (2011)
1) Synthesis, Structure and Reactivities of Porphyrins and Metalloporphyrins
2) Preparation of Hemoproteins having Non-natural Prosthetic Groups
3) Physicochemical Properties of Tetrapyrrole Macrocycles and Their Metal Complexes
4) Improvement and/or Conversion of Hemoprotein and Nonhemoprotein Functions
5) Elucidation of Molecular Mechanism of Hemoproteins
6) Supramolecular Assembly of Hemoproteins
7) Practical Application of Bioinorganic Chemistry
- Selected Papers (30 papers)
1. Molecular Recognition of Ubiquinone Analogues. Specific Interaction between Quinone and Functional Porphyrin via Multi-Hydrogen-Bonding" Takashi Hayashi,* Takashi Miyahara, Norihiro Koide, Yukitoshi Kato, Hideki Masuda, Hisanobu Ogoshi* J. Am. Chem. Soc., 119, 7281–7290 (1997). 2. Artificial Protein-Protein Complexation between Reconstituted Myoglobin and Cytochrome c Takashi Hayashi,* Yutaka Hitomi, Hisanobu Ogoshi* J. Am. Chem. Soc., 120, 4910–4915 (1998). 3. Peroxidase Activity of Myoglobin is Enhanced by Chemical Mutation of Heme-Propionates Takashi Hayashi,* Yutaka Hitomi, Tsutomu Ando, Tadashi Mizutani, Yoshio Hisaeda, Susumu Kitagawa, Hisanobu Ogoshi J. Am. Chem. Soc., 121, 7747–7750 (1999). 4. Interprotein Electron Transfer Reaction Regulated by an Artificial Interface Yutaka Hitomi, Takashi Hayashi,* Kenji Wada, Tadashi Mizutani, Yoshio Hisaeda, Hisanobu Ogoshi Angew. Chem. Int. Ed., 40, 1098–1101 (2001). 5. First Catalysis of Cobaltporphycenes. Oxidation of Vinyl Ether via Formation and Dissociation of Cobalt-Carbon Bond Takashi Hayashi,* Kenshi Okazaki, Naomi Urakawa, Hisashi Shimakoshi, Jonathan L. Sessler, Emanuel Vogel, Yoshio Hisaeda Organometallics, 20, 3074–3078 (2001). 6. Blue Myoglobin Reconstituted with an Iron Porphycene Shows Extremely High Oxygen Affinity Takashi Hayashi,* Hirohisa Dejima, Takashi Matsuo, Hideaki Sato, Yoshio Hisaeda* J. Am. Chem. Soc., 124, 11226–11227 (2002). 7. Reductive Activation of Dioxygen by Myoglobin: Reactivity of Myoglobin Reconstituted with Flavohemin Takashi Matsuo, Takashi Hayashi,* Yoshio Hisaeda J. Am. Chem. Soc., 124, 11234–11235 (2002). 8. Synthesis, Structure and Chemical Property of a First Fluorine-Containing Porphycene Takashi Hayashi,* Yuji Nakashima, Kazuyuki Ito, Takahiro Ikegami, Isao Aritome, Akihiko Suzuki, Yoshio Hisaeda* Org. Lett., 5, 2845–2848 (2003). 9. Synthesis, Characterization, and Autoreduction of a Highly Electron-Deficient Porphycenatoiron(III) with Trifluoromethyl Substituents Takashi Hayashi,* Yuji Nakashima, Kazuyuki Ito, Takahiro Ikegami, Isao Aritome, Tsutomu Ando, Katsuhiro Aoyagi, Yoshio Hisaeda* Inorg. Chem., 23, 7345–7347 (2003). 10. Hybridization of Modified-Heme Reconstitution and Distal Histidine Mutation to Functionalize Sperm Whale Myoglobin Hideaki Sato, Takashi Hayashi,* Tsutomu Ando, Yoshio Hisaeda, Takafumi Ueno, Yoshihito Watanabe J. Am. Chem. Soc., 126, 436–437 (2004). 11. Ligand Binding Properties of Myoglobin Reconstituted with Iron Porphycene: Unusual O2 Binding Selectivity against CO Binding Takashi Matsuo, Hirohisa Dejima, Shun Hirota, Dai Murata, Hideaki Sato, Takahiro Ikegami, Hiroshi Hori, Yoshio Hisaeda, Takashi Hayashi* J. Am. Chem. Soc., 126, 16007–16017 (2004). 12. Unusual Ligand Discrimination by a Myoglobin Reconstituted with a Hydrophobic Domain-Linked Heme Hideaki Sato, Masahiro Watanabe, Yoshio Hisaeda, Takashi Hayashi* J. Am. Chem. Soc., 127, 56–57 (2005). 13. Preparation and O2 Binding Study of Myoglobin Having a Cobalt Porphycene Takashi Matsuo, Takashi Tsuruta, Keiko Maehara, Hideaki Sato, Yoshio Hisaeda, Takashi Hayashi* Inorg. Chem., 44, 9391–9396 (2005). 14. Crystal Structure and Peroxidase Activity of Myoglobin Reconstituted with Iron Porphycene Takashi Hayashi*, Dai Murata, Masatomo Makino, Hiroshi Sugimoto, Takashi Matsuo, Hideaki Sato, Yoshitsugu Shiro, Yoshio Hisaeda* Inorg. Chem., 45, 10530–10536 (2006). 15. Construction of Glycosylated Myoglobin by Reconstitutional Method Takashi Matsuo, Hirokazu Nagai, Yoshio Hisaeda, Takashi Hayashi* Chem. Commun., 3131–3133 (2006). 16. Supramolecular Hemoprotein Linear Assembly by Successive interprotein Heme--Heme Pocket Interactions Hiroaki Kitagishi, Koji Oohora, Hiroyasu Yamaguchi, Hideki Sato, Takashi Matsuo, Akira Harada, Takashi Hayashi* J. Am. Chem. Soc., 129, 10326–10327 (2007). 17. Porphyrinoid Chemistry in Hemoprotein Matrix: Detection and Reactivities of Iron(IV)-Oxo Species of Porphycene Incorporated into Horseradish Peroxidase Takashi Matsuo, Dai Murata, Yoshio Hisaeda, Hiroshi Hori, Takashi Hayashi* J. Am. Chem. Soc., 129, 12906–12907 (2007). 18. Structure and Ligand Binding Properties of Myoglobins Reconstituted with Monodepropionated Heme: Functional Role of Each Heme Propionate Side Chain Katsuyoshi Harada, Masatomo Makino, Hiroshi Sugimoto, Shun Hirota, Takashi Matsuo, Yoshitsugu Shiro, Yoshio Hisaeda, Takashi Hayashi* Biochemistry, 46, 9406–9416 (2007). 19. A Structural Isomer of Nonaromatic Porphyrin: Preparation of 20π-Conjugated Porphycene Based on Electronic Perturbation Takashi Matsuo, Kazuyuki Ito, Nobuko Kanehisa, Takashi Hayashi* Org. Lett., 9, 5303–5306 (2007). 20. Evaluation of the Funcitonal Role of the Heme-6-propionate Side Chain in Cytochrome P450cam Katsuyoshi Harada, Keisuke Sakurai, Kenichiro Ikemura, Takashi Ogura, Shun Hirota, Hideo Shimada*, Takashi Hayashi* J. Am. Chem. Soc., 130, 432–433 (2008). 21. Photocatalytic Hydrogen Generation Using a Protein-Coated Photosensitizer with Anionic Patches and a Monocationic Electron Mediator Takashi Matsuo, Atsushi Asano, Tsutomu Ando, Yoshio Hisaeda, Takashi Hayashi* Chem. Commun., 31, 3684–3686, (2008). 22. A Role of the Heme-7-Propionate Side Chain in Cytochrome P450cam as a Gate for Regulating the Access of Water Molecules to the Substrate-Binding Site Takashi Hayashi,* Katsuyoshi Harada, Keisuke Sakurai, Hideo Shimada, Shun Hirota J. Am. Chem. Soc., 4, 1398–1400 (2009). 23. Self-Assembly of One- and Two-Dimensional Hemoprotein Systems by Polymerization through Heme-Heme Pocket Interactions Hiroaki Kitagishi, Yasuaki Kakikura, Hiroyasu Yamaguchi, Koji Oohora, Akira Harada, Takashi Hayashi* Angew. Chem. Int. Ed., 48, 1271–1274 (2009). Inside Front Cover 24. Meso-Unsubstituted Iron Corrole in Hemoproteins: Remarkable Differences in Effects on Peroxidase Activities between Myoglobin and Horseradish Peroxidase Takashi Matsuo*, Akihiro Hayashi, Masato Abe, Takaaki Matsuda, Yoshio Hisaeda, Takashi Hayashi* J. Am. Chem. Soc., 131, 15124–15125 (2009). 25. Supramolecular Hemoprotein-Gold Nanoparticle Conjugates Akira Onoda, Yuichi Ueya, Taiki Sakamoto, Taro Uematsu, Takashi Hayashi* Chem. Commun., 46, 9107–9109 (2010). Front Cover 26. A Chemically-controlled Supramolecular Protein Polymer Formed by a Myoglobin-based Self-assembly System Koji Oohora, Akira Onoda, Hiroaki Kitagishi, Hiroyasu Yamaguchi, Akira Harada, Takashi Hayashi* Chem. Sci., 2, 1033–1038, (2011). Backside Cover 27. Crystal Structure and Spectroscopic Studies of a Stable Mixed-Valent State of the Hemerythrin-like Domain of a Bacterial Chemotaxis Protein Akira Onoda,* Yasunori Okamoto, Hiroshi Sugimoto, Yoshitsugu Shiro, Takashi Hayashi* Inorg. Chem., 50, 4892–4899, (2011). 28. A Hydrogenase Model Ssystem Based on the Sequence of Cytochrome c: Photochemical Hydrogen Evolution in Aqueous Media Yohei Sano, Akira Onoda, Takashi Hayashi* Chem. Commun., 47, 8229–8231 (2011). Inside Front Cover 29. Photocurrent Generation of Hierarchical Zinc-substituted Hemoprotein Assemblies Immobilized on Gold Electrode Akira Onoda, Yasuaki Kakikura, Taro Uematsu, Susumu Kuwabata, Takashi Hayashi* Angew. Chem. Int. Ed., 51, 2628–2631 (2012). 30. Chemically Programmed Supramolecular Assembly of Hemoprotein and Streptavidin with Alternating Alignment Koji Oohora, Sabina Burazerovic, Akira Onoda, Yvonne M. Wilson, Thomas R. Ward,* Takashi Hayashi* Angew. Chem., Int Ed., 51, 3818–3821 (2012). Front Cover
- Selected Reviews (2 papers)
1. Molecular Modelling of Electron Transfer Systems by Noncovalently Linked Porphyrin-Acceptor Pairing
Takashi Hayashi,* Hisanobu Ogoshi*
Chem. Soc. Rev., 26, 355-364 (1997).
2. New Functionalization of Myoglobin by Chemical Modification of Heme-Propionates
Takashi Hayashi,* Yoshio Hisaeda
Acc. Chem. Res., 35, 35-43 (2002).
- Selected Book Chapters (5 books)
1. Porphyrins and Metalloporphyrins as Receptor Models in Molecular Recognition
Hisanobu Ogoshi, Tadashi Mizutani, Takashi Hayashi, Yasuhisa Kuroda
In The Porphyrin Handbook , K. Kadish, K. M. Smith, R. Guilard, Eds., Vol. 6, Chapt 46, 279–340, Academic Press, San Diego, (1997).
2. Zinc-Containing Enzymes and Their Models
In Encyclopedia of Supramolecular Chemistry , J. Atwood, J. Steed, Eds. Marcel Dekker, New York, 1631–1638 (2004).
3. Iron: Heme Proteins & Dioxygen Transport & Storage
In Encyclopedia of Inorganic Chemistry 2, Bioinorganic Chemistry , R. B. King, Ed. John Wiley & Sons, Chichester, 2167–2180 (2005).
4. Functionalization of Myoglobin
Yoshihito Watanabe, Takashi Hayashi
In Progress in Inorganic Chemistry , Vol. 54. K. Karlin, Ed. Chapt.7 449–493 (2005).
5. Hemoproteins Reconstituted with Artificially Created Hemes
In Handbook of Porphyrin Science 5, Heme Proteins, K. M Kadish, K. M. Smith, R. Guilard, Eds., World Scientific, Singapore, 1–69 (2010).
- Selected Invited Presentations (From 2005)
PACIFICHEM 2005, ---Designed Protein Alterations and Their Applications (#130)
Honolulu, USA, December 2005 [Invited Lecture]
"Functionalization of Hemoproteins by Reconstitutional Method Using a Non-native Heme"
PACIFICHEM 2005, ---The Role of Metals in Metalloenzymes and Medicine (#81)
Honolulu, USA, December 2005 [Invited Lecture]
"Physicochemical Property of Myoglobin with an Iron Porphycene as a Prosthetic Group"
4th International Conference on Porphyrins and Phthalocyanines (ICPP-4)
Rome, Italy, July, 2006. [Invited Lecture]
"Metalloporphycene is an Attractive Prosthetic Group for Hemoproteins"
Gordon Research Conferences; Chemistry & Biology of Tetrapyrroles,
Newport, USA, July 2006 [Invited Lecture]
"Evaluation of Heme-Proteins Interaction by Artificially Created Prosthetic Groups"
2nd International Symposium on Biomolecular Chemistry (ISBC2006)
Kobe, August 2006 [Invited Lecture]
"Functionalization of Hemoproteins by Chemical Modification of Prosthetic Groups"
International Symposium on Bioinorganic Chemistry of the New Age
Suita, October, 2006 [Invited Lecture]
"Physicochemical Properties of Reconstituted Hemoproteins Having Monoproionated Hemin"
12th IUPAC International Symposium on MacroMolecular Complexes (MMC-12)
Fukuoka, August, 2007 [Invited Lecture]
"Supramolecular Polymerization of Hemoproteins to Construct Unique Linear Assembly Biomaterials"
France-Japan Collaboration Supported by CNRS and JSPS. 7th Workshop at Strasbourg
Strasbourg, France, September 2007 [Invited Lecture]
"Insertion of Artificially Created Heme into Apoprotein to Functionalize Hemoprotein"
67th Okazaki Conference. "Molecular Science and Chemical Biology of Biomolecular Function"
Okazaki, November, 2007 [Invited Lecture]
"Role of Heme-Propionate Side Chains in Cytochrome P450cam: Each Propionate is Essential to Retaining the Enzyme Activity"
5th International Conference on Porphyrins and Phthalocyanines (ICPP-5)
Moscow Russia, July 2008 [Invited Lecture]
"Enhancement of Peroxidase Activity for Several Hemoproteins by Reconstitution with Artificially Created Prosthetic Groups"
The 4th Asian Biological Inorganic Chemistry Conference (AsBIC-4)
Jeju Korea, November, 2008 [invited Lecture]
"Evaluation of Functional Role of Heme-Propionate Side Chains in Cytochrome P450cam"
The IUMRS International Conference in Asia 2008
Nagoya, December, 2008 [Invited Lecture]
"Construction of Supramolecular Polymer Formed by Hemoprotein Assembly"
The 2nd Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC2)
Parry Sound, Canada [Invited Lecture]
“Physicochemical Property and Catalytic Activity of Hemoprotein Having a Non-natural Tetrapyrrole Iron Complex as a Prosthetic Group”
14th International Conference on Biological Inorganic Chemistry (ICBIC-14)
Nagoya, July 2009 [Invited Lecture]
“Molecular Design of Prosthetic Group in Hemoproteins"
The 2nd Japan-Korea Seminar on Biomolecular Sciences-Experiments and Simulations
Nagoya, December, 2009 [Invited Lecture]
“A Role of Heme-7-Propionate Side Chain in Cytochrome P450cam"
Chemistry at the Frontiers of Biology and Physics (Osaka Univ. GCOE–Univ. Strasbourg Joint Symposium)
Strasbourg, France, July 2010 [Invited Lecture]
“Supramolecular hemoprotein polymer formed by interprotein heme–heme pocket interaction"
6th International Conference on Porphyrins and Phthalocyanines (ICPP-6)
Santa Ana Pueblo, USA, July 2010 [Invited Lecture]
“Preparation and Characterization of Supramolecular Hemoprotein Polymers Through Heme-Heme Pocket Interaction"
3rd Aachen-Osaka Joint Symposium
Aachen, Germany, September 2010 [Invited Lecture]
“Enhancement of Enzymatic Activities of Myoglobin and Horseradish Peroxidase by Heme-Substitution Methods"
The 5th Asian Biological Inorganic Chemistry Conference (AsBIC V)
Kaohsiung, Taiwan, November, 2010 [Invited Lecture]
“Modification of Hemoproteins to Create Hemoprotein Polymers”
Pacifichem2010, Molecular Design in Bioinorganic Chemistry #173
Honolulu, USA, December 2010 [Invited Lecture]
“Modification of Hemoproteins by Heme-substitution”
The 3rd Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC3)
Parry Sound, Canada [Invited Lecture]
“Water Expelling Mechanism from the Substrate-Binding Site in Cytochrome P450cam”
International Symposium on Activation of Dioxygen and Homogeneous Catalytic Oxidation(ns)
Nago, July 2011 [Invited Lecture]
“Peroxidase Activity of Myoglobin Reconstituted with an Artificially Created Prosthetic Group”
15th International Conference on Biological Inorganic Chemistry (ICBIC-15)
Vancouver, Canada, 2011 [Keynote Lecture]
“Functionalization of Hemoproteins with Artificially Modified Heme”
4th Aachen-Osaka Joint Symposium "Direct Catalytic Activation of Inert Substrates
Aachen, Germany, September 2011 [Invited Lecture]
“New Synthetic Model for [FeFe]-Hydrogenase using Cytochrome c as a Protein Matrix”
3rd Dalton Transactions International Symposium Bioinorganic Chemistry
Suita, November, 2011 [Invited Lecture]
“Construction of Supramolecular Hemoprotein Self-Assembly Systems”
221th Electrochemical Society Meeting (221th ECS Meeting)
Seattle, USA, May 2012 [Invited Lecture]
“Physicochemical Properties of Hemoproteins Reconstituted with Metalloporphyrins”
6th International Conference on Porphyrins and Phthalocyanines (ICPP-6)
Jeju Koria, July 2012 [Invited Lecture]
“Supramolecular Hemoprotein Assembly via Heme–Heme Pocket Interaction”
6th International Symposium on Bioorganometallic Chemistry (ISBOMC’12)
Toronto, Canada, July 2012 [Plenary Lecture]
“Heme Pocket Is an Attractive Cavity for Bioorganometallic Reaction Centers”
6th Asian Conference on Biological Inorganic Chemistry (AsBIC6)
Hong Kong, China, November 2012 [Plenary Lecture]
“Structure and Function of Hemoproteins with Artificially Created Prosthetic Groups”
- Selected Faculty Seminar in Foreign Country (From 2002)
2002 University of Pennsylvania, Philadelphia/USA (October) Johns Hopkins University, Baltimore/USA (October) University of Washington, Seattle/USA (October) 2003 The University of Sydney, Sydney/Australia (July) University of Illinois, Urbana-Champaign, Urbana-Champaign/USA (October) 2006 University of Florence, Florence/Italy (July) 2007 University of British Columbia, Vancouver/Canada (November) University of Texas at Austin, Austin/USA (November) 2008 Academia Sinica, Taipei/Taiwan (January) National Tsing-Hua University, Hsinchu/Taiwan (January) Oregon Health & Science University, Beaverton/USA (March) Scripps Research Institute, La Jolla/USA (March) University of California Irvine, Orange County/USA (March) 2010 University of Strasbourg, Strasbourg/France (March) University of Basel, Basel/Switzerland (March) University of Bourgogne, Dijon/France (March) University of Marseille, Marseille /France (June) CEA, Grenoble/France (June) Technical University of Dortmund, Dortmund/Germany (September) 2011 RWTH, Aachen/Germany (March) ETH, Zurich/Switzerland (March) Technical University of Eindhoven, Eindhoven/The Netherland (September) University of Rennes 1, Rennes/France (September) 2012 University of South Carolina/USA (March) Georgia State University/USA (March) University of Georgia/USA (March) University of Washington/USA (May) University of California Irvine/USA (MAY) Technical University of Braunschweig/Germany (September) 2013 California Institute of Technology/USA (July) University of Strasbourg/France (October) Polish Academy of Sciences/Poland (October)