Department of Applied Chemistry, Graduate School of Engineering, Osaka University
Prof. HAYASHI
Takashi HAYASHIProfessor of Chemistry
- Affiliation
Department of Applied Chemistry,
Graduate School of Engineering,
Osaka University- Address
Building/Room #C4-621
2-1 Yamadaoka, Suita, Osaka 565-0871, Japan- Contact
E-mail: thayashi ”at” chem.eng.osaka-u.ac.jp ( ”at” = @)
Phone: +81-(0)6-6879-7928 Fax: +81-(0)6-6879-7930
Career
- Education
B. Eng., Kyoto University, March 1985
M. Eng., Kyoto University, March 1987(supervisor: Prof. Iwao Tabushi)
Ph.D. in Eng., Kyoto University, March 1990(supervior: Prof. Yoshihiko Ito)- Professional Career
Assistant Professor, Faculty of Engineering, Kyoto University, 1990–1997
Visiting Scientist, The Scripps Research Institute, 1995–1996
Associate Professor, Faculty of Engineering, Kyushu University, 1997–2005
Project Leader on Precursory Research for Embryonic Science and Technology (PREST)
in Japan Science and Technology Corporation (JST), 2000–2003
Professor, Faculty of Engineering, Osaka University, 2005–present
Visiting Professor, Institute of Molecular Science, 2005–2007
Visiting Professor, University of Strasbourg, 2010.
Activities
- Memberships
Chemical Society of Japan (CSJ),
American Chemical Society (ACS),
Japan Society of Coordination Chemistry (JSCC),
Society of Biological Inorganic Chemistry (SBIC),
Society of Porphyrins and Phthalocyanines (SPP),
Society of Synthetic Organic Chemistry of Japan,
Society of Polymer Science of Japan,
Catalysis Society of Japan,
The Biophysical Society of Japan.- Council Member
Division of the Biofunctional Chemistry and Biotechnology, CSJ, 2006-2010
Japan Society of Coordination Chemistry, JSCC, 2009-2011
Vice-president, Japan Society of Coordination Chemistry, JSCC, 2018-2020
Kinki Chemical Society, 2019- current
JSPS Program officer of Research Center for Science Systems, 2022- current- Editorial Board Member
J. Porphyrins Phthalocyanines,
J. Inorg. Biochem.
Bulletin of Chemical Society of Japan (BCSJ) Associate Editor, 2005–2011
J. Biol. Inorg. Chem.- Awards
Kaneka Corporation Award in Synthetic Organic Chemistry (1992)
Inoue Research Award for Young Scientists (1992)
The Progress Award on Synthetic Organic Chemistry, Japan (2000)
1st Young Investigator Award in Porphyrin Chemistry (2000)
2001 Kyushu University President Award (2002)
7th Research Promoted Award on Enzyme Application supported by Amano Enzyme Inc. (2006)
BCSJ Award (2008)
The 27th Chemical Society of Japan Award for Creative Work (2010)
Osaka University Achievement Awards in Research (2011)
Humboldt Research Award (2022)
Research Interests
1) Synthesis, Structure and Reactivities of Porphyrins and Metalloporphyrins
2) Preparation of Hemoproteins having Non-natural Prosthetic Groups
3) Physicochemical Properties of Tetrapyrrole Macrocycles and Their Metal Complexes
4) Improvement and/or Conversion of Hemoprotein and Nonhemoprotein Functions
5) Elucidation of Molecular Mechanism of Hemoproteins
6) Supramolecular Assembly of Hemoproteins
7) Practical Application of Bioinorganic Chemistry
Selected Papers
- Selected Papers (50 papers)
1. Molecular Recognition of Ubiquinone Analogues. Specific Interaction between Quinone and Functional Porphyrin via Multi-Hydrogen-Bonding" Takashi Hayashi,* Takashi Miyahara, Norihiro Koide, Yukitoshi Kato, Hideki Masuda, Hisanobu Ogoshi* J. Am. Chem. Soc., 119, 7281–7290 (1997). 2. Artificial Protein-Protein Complexation between Reconstituted Myoglobin and Cytochrome c Takashi Hayashi,* Yutaka Hitomi, Hisanobu Ogoshi* J. Am. Chem. Soc., 120, 4910–4915 (1998). 3. Peroxidase Activity of Myoglobin is Enhanced by Chemical Mutation of Heme-Propionates Takashi Hayashi,* Yutaka Hitomi, Tsutomu Ando, Tadashi Mizutani, Yoshio Hisaeda, Susumu Kitagawa, Hisanobu Ogoshi J. Am. Chem. Soc., 121, 7747–7750 (1999). 4. Interprotein Electron Transfer Reaction Regulated by an Artificial Interface Yutaka Hitomi, Takashi Hayashi,* Kenji Wada, Tadashi Mizutani, Yoshio Hisaeda, Hisanobu Ogoshi Angew. Chem. Int. Ed., 40, 1098–1101 (2001). 5. First Catalysis of Cobaltporphycenes. Oxidation of Vinyl Ether via Formation and Dissociation of Cobalt-Carbon Bond Takashi Hayashi,* Kenshi Okazaki, Naomi Urakawa, Hisashi Shimakoshi, Jonathan L. Sessler, Emanuel Vogel, Yoshio Hisaeda Organometallics, 20, 3074–3078 (2001). 6. Blue Myoglobin Reconstituted with an Iron Porphycene Shows Extremely High Oxygen Affinity Takashi Hayashi,* Hirohisa Dejima, Takashi Matsuo, Hideaki Sato, Yoshio Hisaeda* J. Am. Chem. Soc., 124, 11226–11227 (2002). 7. Reductive Activation of Dioxygen by Myoglobin: Reactivity of Myoglobin Reconstituted with Flavohemin Takashi Matsuo, Takashi Hayashi,* Yoshio Hisaeda J. Am. Chem. Soc., 124, 11234–11235 (2002). 8. Synthesis, Structure and Chemical Property of a First Fluorine-Containing Porphycene Takashi Hayashi,* Yuji Nakashima, Kazuyuki Ito, Takahiro Ikegami, Isao Aritome, Akihiko Suzuki, Yoshio Hisaeda* Org. Lett., 5, 2845–2848 (2003). 9. Synthesis, Characterization, and Autoreduction of a Highly Electron-Deficient Porphycenatoiron(III) with Trifluoromethyl Substituents Takashi Hayashi,* Yuji Nakashima, Kazuyuki Ito, Takahiro Ikegami, Isao Aritome, Tsutomu Ando, Katsuhiro Aoyagi, Yoshio Hisaeda* Inorg. Chem., 23, 7345–7347 (2003). 10. Hybridization of Modified-Heme Reconstitution and Distal Histidine Mutation to Functionalize Sperm Whale Myoglobin Hideaki Sato, Takashi Hayashi,* Tsutomu Ando, Yoshio Hisaeda, Takafumi Ueno, Yoshihito Watanabe J. Am. Chem. Soc., 126, 436–437 (2004). 11. Ligand Binding Properties of Myoglobin Reconstituted with Iron Porphycene: Unusual O2 Binding Selectivity against CO Binding Takashi Matsuo, Hirohisa Dejima, Shun Hirota, Dai Murata, Hideaki Sato, Takahiro Ikegami, Hiroshi Hori, Yoshio Hisaeda, Takashi Hayashi* J. Am. Chem. Soc., 126, 16007–16017 (2004). 12. Unusual Ligand Discrimination by a Myoglobin Reconstituted with a Hydrophobic Domain-Linked Heme Hideaki Sato, Masahiro Watanabe, Yoshio Hisaeda, Takashi Hayashi* J. Am. Chem. Soc., 127, 56–57 (2005). 13. Preparation and O2 Binding Study of Myoglobin Having a Cobalt Porphycene Takashi Matsuo, Takashi Tsuruta, Keiko Maehara, Hideaki Sato, Yoshio Hisaeda, Takashi Hayashi* Inorg. Chem., 44, 9391–9396 (2005). 14. Crystal Structure and Peroxidase Activity of Myoglobin Reconstituted with Iron Porphycene Takashi Hayashi*, Dai Murata, Masatomo Makino, Hiroshi Sugimoto, Takashi Matsuo, Hideaki Sato, Yoshitsugu Shiro, Yoshio Hisaeda* Inorg. Chem., 45, 10530–10536 (2006). 15. Construction of Glycosylated Myoglobin by Reconstitutional Method Takashi Matsuo, Hirokazu Nagai, Yoshio Hisaeda, Takashi Hayashi* Chem. Commun., 3131–3133 (2006). 16. Supramolecular Hemoprotein Linear Assembly by Successive interprotein Heme--Heme Pocket Interactions Hiroaki Kitagishi, Koji Oohora, Hiroyasu Yamaguchi, Hideki Sato, Takashi Matsuo, Akira Harada, Takashi Hayashi* J. Am. Chem. Soc., 129, 10326–10327 (2007). 17. Porphyrinoid Chemistry in Hemoprotein Matrix: Detection and Reactivities of Iron(IV)-Oxo Species of Porphycene Incorporated into Horseradish Peroxidase Takashi Matsuo, Dai Murata, Yoshio Hisaeda, Hiroshi Hori, Takashi Hayashi* J. Am. Chem. Soc., 129, 12906–12907 (2007). 18. Structure and Ligand Binding Properties of Myoglobins Reconstituted with Monodepropionated Heme: Functional Role of Each Heme Propionate Side Chain Katsuyoshi Harada, Masatomo Makino, Hiroshi Sugimoto, Shun Hirota, Takashi Matsuo, Yoshitsugu Shiro, Yoshio Hisaeda, Takashi Hayashi* Biochemistry, 46, 9406–9416 (2007). 19. A Structural Isomer of Nonaromatic Porphyrin: Preparation of 20π-Conjugated Porphycene Based on Electronic Perturbation Takashi Matsuo, Kazuyuki Ito, Nobuko Kanehisa, Takashi Hayashi* Org. Lett., 9, 5303–5306 (2007). 20. Evaluation of the Funcitonal Role of the Heme-6-propionate Side Chain in Cytochrome P450cam Katsuyoshi Harada, Keisuke Sakurai, Kenichiro Ikemura, Takashi Ogura, Shun Hirota, Hideo Shimada*, Takashi Hayashi* J. Am. Chem. Soc., 130, 432–433 (2008). 21. Photocatalytic Hydrogen Generation Using a Protein-Coated Photosensitizer with Anionic Patches and a Monocationic Electron Mediator Takashi Matsuo, Atsushi Asano, Tsutomu Ando, Yoshio Hisaeda, Takashi Hayashi* Chem. Commun., 31, 3684–3686, (2008). 22. A Role of the Heme-7-Propionate Side Chain in Cytochrome P450cam as a Gate for Regulating the Access of Water Molecules to the Substrate-Binding Site Takashi Hayashi,* Katsuyoshi Harada, Keisuke Sakurai, Hideo Shimada, Shun Hirota J. Am. Chem. Soc., 4, 1398–1400 (2009). 23. Self-Assembly of One- and Two-Dimensional Hemoprotein Systems by Polymerization through Heme-Heme Pocket Interactions Hiroaki Kitagishi, Yasuaki Kakikura, Hiroyasu Yamaguchi, Koji Oohora, Akira Harada, Takashi Hayashi* Angew. Chem. Int. Ed., 48, 1271–1274 (2009). Inside Front Cover 24. Meso-Unsubstituted Iron Corrole in Hemoproteins: Remarkable Differences in Effects on Peroxidase Activities between Myoglobin and Horseradish Peroxidase Takashi Matsuo*, Akihiro Hayashi, Masato Abe, Takaaki Matsuda, Yoshio Hisaeda, Takashi Hayashi* J. Am. Chem. Soc., 131, 15124–15125 (2009). 25. Supramolecular Hemoprotein-Gold Nanoparticle Conjugates Akira Onoda, Yuichi Ueya, Taiki Sakamoto, Taro Uematsu, Takashi Hayashi* Chem. Commun., 46, 9107–9109 (2010). Front Cover 26. A Chemically-controlled Supramolecular Protein Polymer Formed by a Myoglobin-based Self-assembly System Koji Oohora, Akira Onoda, Hiroaki Kitagishi, Hiroyasu Yamaguchi, Akira Harada, Takashi Hayashi* Chem. Sci., 2, 1033–1038, (2011). Backside Cover 27. Crystal Structure and Spectroscopic Studies of a Stable Mixed-Valent State of the Hemerythrin-like Domain of a Bacterial Chemotaxis Protein Akira Onoda,* Yasunori Okamoto, Hiroshi Sugimoto, Yoshitsugu Shiro, Takashi Hayashi* Inorg. Chem., 50, 4892–4899, (2011). 28. A Hydrogenase Model Ssystem Based on the Sequence of Cytochrome c: Photochemical Hydrogen Evolution in Aqueous Media Yohei Sano, Akira Onoda, Takashi Hayashi* Chem. Commun., 47, 8229–8231 (2011). Inside Front Cover 29. Photocurrent Generation of Hierarchical Zinc-substituted Hemoprotein Assemblies Immobilized on Gold Electrode Akira Onoda, Yasuaki Kakikura, Taro Uematsu, Susumu Kuwabata, Takashi Hayashi* Angew. Chem. Int. Ed., 51, 2628–2631 (2012). 30. Chemically Programmed Supramolecular Assembly of Hemoprotein and Streptavidin with Alternating Alignment Koji Oohora, Sabina Burazerovic, Akira Onoda, Yvonne M. Wilson, Thomas R. Ward,* Takashi Hayashi* Angew. Chem., Int Ed., 51, 3818–3821 (2012). Front Cover
31. H2O2-dependent Substrate Oxidation by an Engineered Diiron Site in a Bacterial Hemerythrin
Yasunori Okamoto, Akira Onoda,* Hiroshi Sugimoto, Yu Takano, Shun Hirota, Donald M. Kurtz, Jr., Yoshitsugu Shiro, Takashi Hayashi*
Chem. Commun., 50, 3421-3423 (2014). Front Cover
32. C(sp3)-H Bond Hydroxylation Catalyzed by Myoglobin Reconstituted with Manganese Porphycene
Koji Oohora, Yushi Kihara, Eiichi Mizohata, Tsuyoshi Inoue, Takashi Hayashi*
J. Am. Chem. Soc., 135, 17282-17285 (2013).
33. A Rhodium Complex-linked Hybrid Biocatalyst: Stereo-controlled Phenylacetylene Polymerization within an Engineered Protein Cavity
Kazuki Fukumoto, Akira Onoda, Eiichi Mizohata, Marco Bocola, Tsuyoshi, Inoue, Ulrich Schwaneberg, Takashi Hayashi*
ChemCatChem, 6, 1229-1235 (2014). Front Cover
34. Co(II)/Co(I) Reduction-induced Axial Histidine-flipping in Myoglobin Reconstituted with a Cobalt Tetradehydrocorrin as a Methionine Synthase Model
Takashi Hayashi,* Yoshitsugu Morita, Eiichi Mizohata, Koji Oohora, Jun Ohbayashi, Tsuyoshi Inoue, Yoshio Hisaeda
Chem. Commun., 50, 12560-12563 (2014).35. meso-Dibenzoporphycene has a Large Bathochromic Shift and a Porphycene Framework with an Unusual Cis Tautomeric Form
Koji Oohora, Ayumu Ogawa, Tamaki Fukuda, Akira Onoda, Junya Hasegawa,* Takashi Hayashi*
Angew. Chem. Int. Ed., 54, 6227-6230 (2015).36. Intraprotein Transmethylation via a CH3–Co(III) Species in Myoglobin Reconstituted with a Cobalt Corrinoid Complex
Yoshitsugu Morita, Koji Oohora, Akiyoshi Sawada, Kazuki Doitomi, Jun Ohbayashi, Takashi Kamachi, Kazunari Yoshizawa, Yoshio Hisaeda, and Takashi Hayashi*
Dalton Trans., 45, 3277-3284 (2016). Front Cover37. Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis
Hirofumi Harada, Akira Onoda,* Takayuki Uchihashi,* Hiroki Watanabe, Naoki Sunagawa, Masahiro Samejima, Kiyohiko Igarashi,* and Takashi Hayashi*
Chem. Sci., 8, 6561-6565 (2017).38. CuAAC in a Distal Pocket: Metal Active-Template Synthesis of Strapped-Porphyrin [2]Rotaxanes
Yuta Miyazaki, Christophe Kahlfuss, Ayumu Ogawa, Takashi Matsumoto, Jennifer A. Wytko, Koji Oohora, Takashi Hayashi,* and Jean Weiss*
Chem. Eur. J., 23, 13579–13582 (2017). Front Cover39. A Pyrene-linked Cavity within a β-Barrel Protein Promotes an Asymmetric Diels-Alder Reaction
Tomoki Himiyama, Naomasa Taniguchi, Shunsuke Kato, Akira Onoda,* and Takashi Hayashi*
Angew. Chem. Int. Ed., 56, 13618-13622 (2017). Front Cover40. Catalytic Cyclopropanation by Myoglobin Reconstituted with Iron Porphycene: Acceleration of Catalysis due to Rapid Formation of the Carbene Species
Koji Oohora, Hiroyuki Meichin, Liming Zhao, Matthew W. Wolf, Akira Nakayama, Jun-ya Hasegawa,* Nicolaai Lehnert* and Takashi Hayashi*
J. Am. Chem. Soc., 139, 18460–18463 (2017).41. Manganese(V) Porphycene Complex Responsible for Inert C–H Bond Hydroxylation in a Myoglobin Matrix
Koji Oohora,* Hiroyuki Meichin, Yushi Kihira, Hiroshi Sugimoto, Yoshitsugu Shiro, and Takashi Hayashi*
J. Am. Chem. Soc., 139, 18460–18463 (2017).42. Supramolecular Hemoprotein Assembly with a Periodic Structure Showing Heme–Heme Exciton Coupling
Koji Oohora,* Nishiki Fujimaki, Ryota Kajihara, Hiroki Watanabe, Takayuki Uchihashi,* and Takashi Hayashi*
J. Am. Chem. Soc., 140, 10145–10148 (2018).43. Myoglobin Reconstituted with Ni Tetradehydrocorrin as a Methane Generating Model of Methyl‐coenzyme M Reductase
Koji Oohora,* Yuta Miyazaki and Takashi Hayashi*
Angew. Chem. Int. Ed., 58, 13813–13817 (2019).44. Electrochemical CO2 reduction by a cobalt bipyricorrole complex: decrease of an overpotential value derived from monoanionic ligand character of the porphyrinoid species
Ayumu Ogawa, Koji Oohora,* Wenting Gu and Takashi Hayashi*
Chem. Commun., 55, 493–496 (2019).45. A ring-shaped hemoprotein trimer thermodynamically controlled by the supramolecular heme–heme pocket interaction
Koji Oohora,* Ryota Kajihara, Nishiki Fujimaki, Takayuki Uchihashi* and Takashi Hayashi*
Chem. Commun., 55, 1544–1547 (2019). Front Cover46. Triazolecarbaldehyde Reagents for One-step N-Terminal Protein Modification
Akira Onoda,* Nozomu Inoue, Eigo Sumiyoshi and Takashi Hayashi*
ChemBioChem, 21, 1274–1278 (2020). Front Cover47. Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-harvesting System
Shota Hirayama, Koji Oohora,* Takayuki Uchihashi* and Takashi Hayashi*
J. Am. Chem. Soc., 142, 1822-1831 (2020).48. Directed Evolution of a Cp*RhIII-Linked Biohybrid Catalyst Based on a Screening Platform with Affinity Purification
Shunsuke Kato, Akira Onoda,* Naomasa Taniguchi, Ulrich Schwaneberg, Takashi Hayashi*
ChemBioChem, 22, 679–685 (2021).49. Evolutionary Engineering of a Cp*Rh(III) Complex-Linked Artificial Metalloenzyme with a Chimeric β-Bareel Protein Scaffold
Shunsuke Kato, Akira Onoda*, Ulrich Schwaneberg, and Takashi Hayashi*
J. Am. Chem. Soc., 145, 8285-8290 (2023).50. Chitin- and Streptavidin-Mediated Affinity Purification Systems: A Screening Platform for Enzyme Discovery
Shunsuke Kato,* Koki Takeuchi, Motonao Iwaki, Kentaro Miyazaki, Kohsuke Honda and Takashi Hayashi*
Angew. Chem. Int. Ed. 62, e20230376 (2023).- Selected Reviews (8 papers)
1. Molecular Modelling of Electron Transfer Systems by Noncovalently Linked Porphyrin-Acceptor Pairing Takashi Hayashi,* Hisanobu Ogoshi* Chem. Soc. Rev., 26, 355-364 (1997). 2. New Functionalization of Myoglobin by Chemical Modification of Heme-Propionates Takashi Hayashi,* Yoshio Hisaeda Acc. Chem. Res., 35, 35-43 (2002). 3. Supramolecular Assembling Systems Formed by Heme-heme Pocket Interactions in Hemoproteins
Koji Oohora, Akira Onoda, Takashi Hayashi*
Chem. Commun., 48, 11714–11726 (2012). Feature Article4. Hemoprotein-based Supramolecular Assembling Systems
Koji Oohora, Takashi Hayashi*
Cur. Opin. Chem. Biol., 19, 154–161 (2014).5. Artficial Hydrogenase: Biommimetic Approaches Controlling Active Molecular Catalysts
Akira Onoda,* Takashi Hayashi*
Cur. Opin. Chem. Biol., 25, 133–140 (2015).6. Hemoproteins Reconstituted with Artificial Metal Complexes as Biohybrid Catalysts
Koji Oohora, Akira Onoda and Takashi Hayashi*
Acc. Chem. Res., 52, 945–954 (2019).7. Myoglobins engineered with artificial cofactors serve as artificial metalloenzymes and models of natural enzymes
Koji Oohora* and Takashi Hayashi*
Dalton Trans., 50, 1940–1949 (2021).8. Focusing on a nickel hydrocorphinoid in a protein matrix: methane generation by methyl-coenzyme M reductase with F430 cofactor and its models
Yuta Miyazaki, Koji Oohora* and Takashi Hayashi*
Chem. Soc. Rev., 51, 1629–1639 (2022)- Selected Book Chapters (8 books)
1. Porphyrins and Metalloporphyrins as Receptor Models in Molecular Recognition Hisanobu Ogoshi, Tadashi Mizutani, Takashi Hayashi, Yasuhisa Kuroda In The Porphyrin Handbook, K. Kadish, K. M. Smith, R. Guilard, Eds., Vol. 6, Chapt 46, 279–340, Academic Press, San Diego, (1997). 2. Iron: Heme Proteins & Dioxygen Transport & Storage Takashi Hayashi In Encyclopedia of Inorganic Chemistry 2, Bioinorganic Chemistry, R. B. King, Ed. John Wiley & Sons, Chichester, 2167–2180 (2005). 3. Functionalization of Myoglobin Yoshihito Watanabe, Takashi Hayashi In Progress in Inorganic Chemistry, Vol. 54. K. Karlin, Ed. Chapt.7 449–493 (2005). 4. Hemoproteins Reconstituted with Artificially Created Hemes Takashi Hayashi In Handbook of Porphyrin Science 5, Heme Proteins, K. M Kadish, K. M. Smith, R. Guilard, Eds., World Scientific, Singapore, 1–69 (2010). 5. Generation of Functionalized Biomolecules Using Hemoprotein Matrices with Small Protein cavities for Incorporation of Cofactors Takashi Hayashi In Coordination Chemistry in Protein Cages, T. Ueno, Y. Watanabe, Eds., Wiley, Hoboken, 87-110 (2013). 6. Artificially Created Metalloenzyme Consisting of an Organometallic Complex Immobilized to a Protein Matrix
Daniel F. Sauer, Takashi Matsuo, Akira Onoda, Jun Okuda, Takashi Hayashi
In Advances in Bioorganometallic Chemistry, T. Hirao, T. Moriuchi, Eds., Elsevier, Amsterdam, 307–328 (2019).7. Myoglobin Derivatives Reconstituted with Modified Metal Porphyrinoids, as a Structural and Functional Models of the Cytochrome P450 Enzymes
Takashi Hayashi, Koji Oohora
In Dioxygene-dependent Heme Enzymes, M. Ikeda-Saito, E. Raven, Eds., RSC, 63-78 (2019).8. Engineering of hemoproteins
Takashi Hayashi and Sunsuke Kato
In Comprehensive Inorganic Chemistry III, J. Reedijk, K. R. Poeppelmeier, Ed., Elsevier, 215-230 (2023)- Selected Invited Presentations (From 2005)
PACIFICHEM 2005, ---Designed Protein Alterations and Their Applications (#130) Honolulu, USA, December 2005 [Invited Lecture] Functionalization of Hemoproteins by Reconstitutional Method Using a Non-native Heme PACIFICHEM 2005, ---The Role of Metals in Metalloenzymes and Medicine (#81) Honolulu, USA, December 2005 [Invited Lecture] Physicochemical Property of Myoglobin with an Iron Porphycene as a Prosthetic Group 4th International Conference on Porphyrins and Phthalocyanines (ICPP-4) Rome, Italy, July, 2006. [Invited Lecture] Metalloporphycene is an Attractive Prosthetic Group for Hemoproteins Gordon Research Conferences; Chemistry & Biology of Tetrapyrroles, Newport, USA, July 2006 [Invited Lecture] Evaluation of Heme-Proteins Interaction by Artificially Created Prosthetic Groups 2nd International Symposium on Biomolecular Chemistry (ISBC2006) Kobe, August 2006 [Invited Lecture] Functionalization of Hemoproteins by Chemical Modification of Prosthetic Groups International Symposium on Bioinorganic Chemistry of the New Age Suita, October, 2006 [Invited Lecture] Physicochemical Properties of Reconstituted Hemoproteins Having Monoproionated Hemin 12th IUPAC International Symposium on MacroMolecular Complexes (MMC-12) Fukuoka, August, 2007 [Invited Lecture] Supramolecular Polymerization of Hemoproteins to Construct Unique Linear Assembly Biomaterials France-Japan Collaboration Supported by CNRS and JSPS. 7th Workshop at Strasbourg Strasbourg, France, September 2007 [Invited Lecture] Insertion of Artificially Created Heme into Apoprotein to Functionalize Hemoprotein 67th Okazaki Conference. "Molecular Science and Chemical Biology of Biomolecular Function" Okazaki, November, 2007 [Invited Lecture] Role of Heme-Propionate Side Chains in Cytochrome P450cam: Each Propionate is Essential to Retaining the Enzyme Activity 5th International Conference on Porphyrins and Phthalocyanines (ICPP-5) Moscow Russia, July 2008 [Invited Lecture] Enhancement of Peroxidase Activity for Several Hemoproteins by Reconstitution with Artificially Created Prosthetic Groups The 4th Asian Biological Inorganic Chemistry Conference (AsBIC-4) Jeju Korea, November, 2008 [invited Lecture] Evaluation of Functional Role of Heme-Propionate Side Chains in Cytochrome P450cam The IUMRS International Conference in Asia 2008 Nagoya, December, 2008 [Invited Lecture] Construction of Supramolecular Polymer Formed by Hemoprotein Assembly The 2nd Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC2) Parry Sound, Canada [Invited Lecture] “Physicochemical Property and Catalytic Activity of Hemoprotein Having a Non-natural Tetrapyrrole Iron Complex as a Prosthetic Group” 14th International Conference on Biological Inorganic Chemistry (ICBIC-14) Nagoya, July 2009 [Invited Lecture] “Molecular Design of Prosthetic Group in Hemoproteins" The 2nd Japan-Korea Seminar on Biomolecular Sciences-Experiments and Simulations Nagoya, December, 2009 [Invited Lecture] “A Role of Heme-7-Propionate Side Chain in Cytochrome P450cam" Chemistry at the Frontiers of Biology and Physics (Osaka Univ. GCOE–Univ. Strasbourg Joint Symposium) Strasbourg, France, July 2010 [Invited Lecture] “Supramolecular hemoprotein polymer formed by interprotein heme–heme pocket interaction" 6th International Conference on Porphyrins and Phthalocyanines (ICPP-6) Santa Ana Pueblo, USA, July 2010 [Invited Lecture] “Preparation and Characterization of Supramolecular Hemoprotein Polymers Through Heme-Heme Pocket Interaction" 3rd Aachen-Osaka Joint Symposium Aachen, Germany, September 2010 [Invited Lecture] “Enhancement of Enzymatic Activities of Myoglobin and Horseradish Peroxidase by Heme-Substitution Methods" The 5th Asian Biological Inorganic Chemistry Conference (AsBIC V) Kaohsiung, Taiwan, November, 2010 [Invited Lecture] “Modification of Hemoproteins to Create Hemoprotein Polymers” Pacifichem2010, Molecular Design in Bioinorganic Chemistry #173 Honolulu, USA, December 2010 [Invited Lecture] “Modification of Hemoproteins by Heme-substitution” The 3rd Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC3) Parry Sound, Canada [Invited Lecture] “Water Expelling Mechanism from the Substrate-Binding Site in Cytochrome P450cam” International Symposium on Activation of Dioxygen and Homogeneous Catalytic Oxidation(ns) Nago, July 2011 [Invited Lecture] “Peroxidase Activity of Myoglobin Reconstituted with an Artificially Created Prosthetic Group” 15th International Conference on Biological Inorganic Chemistry (ICBIC-15) Vancouver, Canada, 2011 [Keynote Lecture] “Functionalization of Hemoproteins with Artificially Modified Heme” 4th Aachen-Osaka Joint Symposium "Direct Catalytic Activation of Inert Substrates Aachen, Germany, September 2011 [Invited Lecture] “New Synthetic Model for [FeFe]-Hydrogenase using Cytochrome c as a Protein Matrix” 3rd Dalton Transactions International Symposium Bioinorganic Chemistry Suita, November, 2011 [Invited Lecture] “Construction of Supramolecular Hemoprotein Self-Assembly Systems” 221th Electrochemical Society Meeting (221th ECS Meeting) Seattle, USA, May 2012 [Invited Lecture] “Physicochemical Properties of Hemoproteins Reconstituted with Metalloporphyrins” 7th International Conference on Porphyrins and Phthalocyanines (ICPP-7) Jeju Koria, July 2012 [Invited Lecture] “Supramolecular Hemoprotein Assembly via Heme–Heme Pocket Interaction” 12th International Symposium on Bioorganometallic Chemistry (ISBOMC’12) Toronto, Canada, July 2012 [Plenary Lecture] “Heme Pocket Is an Attractive Cavity for Bioorganometallic Reaction Centers” 6th Asian Conference on Biological Inorganic Chemistry (AsBIC6) Hong Kong, China, November 2012 [Plenary Lecture] “Structure and Function of Hemoproteins with Artificially Created Prosthetic Groups” 223rd Electrochemical Society Meeting (223rd ECS Meeting)
Toronto, Canada, May 2013 [Invited Lecture]
“Supramolecular Porphyrin Arrays Mediated by Hemoprotein Matrix”
The 4th Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC4)
Parry Sound, Canada, May 2013 [Invited Lecture]
“Alkane Hydroxylation Catalyzed by Myoglobin Reconstituted with Mn-Porphycene”
16th International Conference on Biological Inorganic Chemistry (ICBIC16)
Grenoble, France, July 2013 [Keynote Lecture]
“Construction of a Methionine Synthase Model by Apomyoglobin-Cobalt Corrin Complex”
4th Asian Conference of Coordination Chemistry (ACCC4)
Jeju, Korea, November 2013 [Invited Lecture]
“Structure and Catalytic Activity of Myoglobin Reconstituted with Mn-Porphycene”
The 2nd Japan-France Coordination Chemistry Symposium 2013
Nara, Japan, November 2013 [Invited Lecture]
“Modification of Heme Protein with an Artificial Metalloporphyrinoid”
Aachen-Osaka Joint Symposium
Aachen, Germany, December 2013 [Invited Lecture]
“A Model of Methionine Synthase: Structure and Function of Myoglobin Reconstituted with a Cobalt Corrinoid”
225th Electrochemical Society Meeting (225th ECS Meeting)
Orlando, USA, May 2014 [Invited Lecture]
“Preparation and Physicochemical Properties of Supramolecular Hemoprotein Assembly via Heme–Heme Pocket Interaction”
8th International Conference on Porphyrins and Phthalocyanines (ICPP8)
Istanbul, Turkey, June 2014 [Invited Lecture]
“Structure and Physicochemical Properties of Supramolecular Hemoprotein Assembly Using a Cytochrome b562 Mutant”
41st International Conference on Coordination Chemistry (ICCC41)
Singapore, Singapore, July 2014 [Invited Lecture]
“Manganese Porphycene Acts as an Artificial Cofactor which Catalyzed a C(sp3)–H Bond Hydroxylation in the Myoglobin Heme Pocket”
Aachen-Osaka Joint Symposium
Aachen, Germany, September 2014 [Invited Lecture]
“Supramolecular Protein Assembly Directed to a Construction of an Artificial Light Harvesting System”
7th Asian Biological Inorganic Chemistry Conference (AsBIC7)
Gold Coast, Australia, December 2014 [Invited Lecture]
“Structural Model of Methionine Synthase by Myoglobin Reconstituted with a Cobalt Corrin Derivative”
The 5th Georgian Bay International Conference on Bioinorganic Chemisty (CanBIC5)
Parry Sound, Canada, May 2015 [Invited Lecture]
“A Structure and Functional Model of Methionine Synthase: Myoglobin with a Tetradehydrocorrin Co Complex”
227th Electrochemical Society Meeting (227th ECS Meeting)
Chicago, USA, May 2015 [Invited Lecture]
“Structure and Physicochemocal Properties of Dibenzoporphycene”13th International Symposium on Applied Bioinorganic Chemistry (ISABC13)
Galway, Ireland, June 2015 [Keynote Lecture]
“Modification of Hemoprotein with an Artificially Created Cofactor to Generate a New Biocatalyst”17th International Conference on Biological Inorganic Chemistry (ICBIC17)
Beijing, China, July 2015 [Keynote Lecture]
“Supramolecular Self-Assembly of Hemoproteins via Heme–Heme Pocket Interaction”IUPAC-2015
Busan, Korea, August 2015 [Invited Lecture]
“Myoglobin Reconstituted with Cobalt Tetradehydrocorrin as a Methionine Synthase Model”250th ACS National Meeting
Boston, USA, August 2015 [Invited Lecture]
“Biohybrid Catalysts Constructed in a Protein Matrix with an Artificial Metal Complex”Aachen-Osaka Joint Symposium
Aachen, Germany, September 2015 [Invited Lecture]
“Construction of a Biohybrid Catalyst Using an Apo-from of Hemoproteins”PACIFICEHM2015
Honolulu, USA, December 2015 [Invited Lecture]
“Artificial Light Harvesting Model by a Hexameric Hemoprotein Reconstituted with Zinc Porphyrinoid”229th Electrochemical Society Meeting (229th ECS Meeting)
San Diego, USA, June 2016 [Invited Lecture]
“Construction of Hemoprotein Assembly via Heme–Heme Pocket Interaction and Its Photochemical Property”9th International Conference on Porphyrins and Phthalocyanines (ICPP9)
Nanjing, China, July 2016 [Invited Lecture]
“Hybrid Model of Cobalamin-Binding Domain in Methionine Synthase: Myoglobin Reconstituted with a Tetradehydrocorrin Cobalt Complex”42nd International Conference on Coordination Chemistry (ICCC42)
Brest, France, July 2016 [Invited Lecture]
“Myoglobin Reconstituted with Cobalt Corrin Derivatives: A Functional Model of Methionine Synthase”13th European Biological Inorganic Chemistry Conference (EuroBIC 13)
Budapest, Hungary, August 2016 [Keynote Lecture]
“Heme Enzyme Models by Myoglobin with Artificial Metalloporphyrinoids”Bioanorganisches Symposium 2016y@RWTH Aachen
Aachen, Germany, September 2016 [Invited Lecture]
“Preparation, Characterization and Reactivity of Myoglobin Reconstituted with Artificial Metalloporphyrinoids”SeleCa Workshop 2016
Aachen, Germany, September 2016 [Invited Lecture]
“A Heme Pocket is an Attractive Scaffold for Constructing a Hybrid Catalyst”68th Southeastern Regional Meeting of the American Chemical Society (SERMACS 2016)
Columbia, USA, October
“Functionalization of Myoglobin by Artificial Metalloporphyrinoids”BIonano 2016
Krakow, Poland, November
“Preparation, Characterization and Photochemical Properties of Supramolecular Hemoprotein Assemblies”8th Asian Biological Inorganic Chemistry Conference (AsBIC8)
Auckland, New Zealand, December 2016 [Keynote Lecture]
“Biohybrid Catalysts with Organometallic Complexes”5th Symposium on Advanced Biological Inorganic Chemistry (SABIC2017)
Kolkata/India, January [Keynote Lecture]
“Myoglobin Reconstituted with Artificial Metalloporphyrinoids as an Enzyme Model”The 6th Georgian Bay International Conference on Bioinorganic Chemisty (CanBIC6)
Parry Sound, Canada, May 2017 [Invited Lecture]
“Myoglobin Reconstituted with Cobalt or Nickel Tetradehydrocorrin as a Cofactor for an Enzyme Model”14th International Symposium on Applied Bioinorganic Chemistry (ISABC14)
Toulouse, France, June 2017 [Plenary Lecture]
“Modification of Metalloproteins to Generate New Biocatalysts”1st Molecular Technology Workshop: “Energy and Electron Transfers in Molecular Engineered Materials”
Strasbourg, France, June 2017 [Invited Lecture]
“Supramolecular Hemoprotein Assembly toward Photoinduced Catalysts”6th Asian Conference of Coordination Chemistry (ACCC6)
Melbourne, Australia, July 2017 [Invited Lecture]
“A Biohybrid Catalyst Using a Heme-Pocket with an Artificial Metallocomplex”18th International Conference on Biological Inorganic Chemistry (ICBIC18)
Florianopolis, Brazil, August 2017 [Keynote Lecture]
“Hemoproteins Reconstituted with Organometallic Complexes as an Artificial Cofactor”Aachen-Osaka Joint Symposium
Aachen, Germany, September 2017 [Invited Lecture]
“Hydroxylation of Innert Alkane Species via C-H Bond Activation Catalyzed by Myoglobin Reconstituted with Manganese Porphycene as an Artificial Cofactor”2017 Bilateral Symposium, Genomics Research center, Academia Sinica & School of Science, Osaka University
Taipei, Taiwan, October, 2017 [Invited Lecture]
“An Artificial Metalloenzyme Consisting of a Protein Matrix and Synthetic Metal Cofactor”2nd International Symposium on Biofunctional Chemistry (ISBC2017)
Uji, December 2017 [Invited Lecture]
“Artificial Metalloenzyme: A Hybrid between an Active Metal Complex and a Protein Having a Unique Cavity”233rd Electrochemical Society Meeting (233rd ECS Meeting)
Seattle, USA, May 2018 [Invited Lecture]
“Preparation and Photochemical Properties of Supramolecular Hemoprotein Assemblies”The Copper Bioinorganic Chemistry Symposium (CuBICS 2018)
Marseille, France, May 2018 [Invited Lecture]
“An Artificially Created Biohybrid Catalyst Promoting Diels-Alder Reaction in the Presence of Cu(II) Ion10th International Conference on Porphyrins and Phthalocyanines (ICPP10)
Munich, Germany, July 2018 [Invited Lecture]
“Hemoproteins Reconstituted with Iron and Manganese Porphycene Complexes”43rd International Conference on Coordination Chemistry (ICCC2018)
Sendai, July 2018 [Keynote Lecture]
“Bioorganometallic Reactions in protein matrices Obtained from Hemoproteins”2018 Frontiers in Chemical Biology (FCB2018)
Yilan, Taiwan, August 2018 [Invited Lecture]
“A New Trend in Bioinorganic Chesmitry: A Biohybrid Catalyst toward an Artificial Metalloenzyme”14th European Biological Inorganic Chemistry Conference (EuroBIC 14)
Birmingham, UK, August [Invited Lecture]
“Biohybrid Catalyst: A Model of [FeFe]-Hydrogenase Using a Diiron Cluster Linked in a beta-Barrel ProteinBIONANO2018
Krakow, Poland, September 2018 [Invited Lecture]
“Supramolecular Hemoprotein Assemblies”Aachen-Osaka Joint Symposium
Aachen, Germany, November 2018 [Invited Lecture]
“Biohybrid Catalysts Using Apohemoprotein Scaffods”The 9th Asian Biological Inorganic Chemistry (AsBIC9)
Singapore, December 2018 [Keynote Lecture]
“Myoglobin Reconstituted with a Corrinoid Metal Complex”The 7th Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC7)
Parry Sound, Canada, May 2019 [Invited Lecture]
“Alkane Hydroxylation by Myoglobin Reconstituted with Metalloporphyrinoid”Artzymes2.0
Basel, Switzerland, August 2019 [Plenary Lecture]
“Hemoprotein Modification Toward Artificial Biocatalyst Generation”18th International Conference on Biological Inorganic Chemistry (ICBIC19)
Interlaken, Switzerland, August 2019 [Keynote Lecture]
“Supramolecular Hemoprotein Assemblies”7th Asian Conference on Coordination Chemistry (ACCC2019)
Kuala Lumpur, Malaysia, October 2019 [Invited Lecture]
“Inert Alkane Hydroxylation Catalyzed by Myoglobin Reconstituted with Manganese Porphycene as an Artificial Cofactor”Israel-Japan Conference, “Molecular Catalysis in the Service of Society”
Haifa, Israel, November 2019 [Invited Lecture]
“Artificial Metalloenzyme Consisting of a Hemoprotein with a Synthetic Cofactor”2019 Korea-Taiwan-Japan Biological Inorganic Chemistry Symposium
Taichung, Taiwan, November 2019 [Invited Lecture]
“A Functional Model of Mehtyl-coenzyme M Reductase Using Myoglobin Reconstituted with a Nickel Porphyrinoid Complex”The 2nd Japan-Germany-Singapore Trilateral Symposium on Precision Synthesis and Catalysis
Nara, December 2019 [Invited Lecture]
“Biohybrid Catalysts by Apoprotein as a Reaction Scaffold”efb2021 (european federation of biotechnology)
virtual conference, May 2021 [Plenary Lecture]
“Artificial Metalloenzymes Consisting of Conjugates between Apohemoproteins and Metal Complexes”eBIC2021 – The SBIC Electronic Biological Inorganic Chemistry Meeting
on-line, July 2021 [Invited Lecture]
“Engineering of Hemoproteins to Create Artificial Metalloenzymes”11th International Conference on Porphyrins and Phthalocyanines (ICPP11)
Virtual Meeting, July 2021 [Invited Lecture]
“Reconstituted Hemoproteins with Ni Corrinoids toward Functional Models of Methane-producing Enzyme”PACIFICEHM2020
on-line, December 2021 [Invited Lecture]
“Conversion of myoglobin to an artificial metalloenzyme”12th International Conference on Porphyrins and Phthalocyanines (ICPP12)
Madrid, July 2022 [Invited Lecture]
“Catalysis in Myoglobins Reconstituted with Artificial Metalloporphyrinoids”8th Asian Conference on Coordination Chemistry (ACCC2022)
Hybrid (Taipei), August 2022 [Invited Lecture]
“Conversion of Myoglobin into Artificial Metalloenzymes”44th International Conference on Coordination Chemistry (ICCC2022)
Rimini, August 2022 [Keynote Lecture]
“Artificial Metalloeyznems Formed by Incorporation of Synthetic Metal Cofactors into Apomyoglobin”The 8th Georgian Bay International Conference on Bioinorganic Chemistry (CanBIC8)
Parry Sound, Canada, May 2023 [Invited Lecture]
“Nitrile Synthesis Catalyzed by Myoglobin Reconstituted with Iron Porphycene”16th International Symposium on Applied Bioinorganic Chemistry (ISABC16)
Ioannina, Greece, June 2023 [Invited Lecture]
“A Model of Aldoxime Dehydratase by Myoglobin”20th International Conference on Biological Inorganic Chemistry (ICBIC20)
Adelaide, Australia, July 2023 [Keynote Lecture]
“Conversion of myoglobin to artificial metalloenzymes”International Symposium on Bioorganometallic Chemistry (ISBOMC2023)
Braunschweig, Germany, September 2023 [Keynote Lecture]
“Hemoproteins reconstituted with artificial cofactors that promote organometallic reactions”- Selected Faculty Seminar in Foreign Country (From 2002)
2002 University of Pennsylvania, Philadelphia/USA (October) Johns Hopkins University, Baltimore/USA (October) University of Washington, Seattle/USA (October) 2003 The University of Sydney, Sydney/Australia (July) University of Illinois, Urbana-Champaign, Urbana-Champaign/USA (October) 2006 University of Florence, Florence/Italy (July) 2007 University of British Columbia, Vancouver/Canada (November) University of Texas at Austin, Austin/USA (November) 2008 Academia Sinica, Taipei/Taiwan (January) National Tsing-Hua University, Hsinchu/Taiwan (January) Oregon Health & Science University, Beaverton/USA (March) Scripps Research Institute, La Jolla/USA (March) University of California Irvine, Orange County/USA (March) 2010 University of Strasbourg, Strasbourg/France (March) University of Basel, Basel/Switzerland (March) University of Bourgogne, Dijon/France (March) University of Marseille, Marseille /France (June) CEA, Grenoble/France (June) Technical University of Dortmund, Dortmund/Germany (September) 2011 RWTH, Aachen/Germany (March) ETH, Zurich/Switzerland (March) Technical University of Eindhoven, Eindhoven/The Netherland (September) University of Rennes 1, Rennes/France (September) 2012 University of South Carolina/USA (March) Georgia State University/USA (March) University of Georgia/USA (March) University of Washington/USA (May) University of California Irvine/USA (May) Technical University of Braunschweig/Germany (September) 2013 California Institute of Technology/USA (July) University of Strasbourg/France (October) Polish Academy of Sciences/Poland (October)
2014
Technion-Israel Institute of Technology/Israel (July)
Hebrew University/Israel (July)
Paris-Sud University/France (September)2015
Aix-Marseille University/France (March)
University of Oxford/UK (June)2016
University of Strasbourg/France (May)
University of Michigan/USA (October)2017
University of Zurich/Switzerland (March)
University of Basel/Switzerland (March)2018
University of Groningen/The Netherlands (September)2019
Max Planck Institute for Chemical Energy Conversion/Germany (February)
University of Napoli/Italy (December)2023
University of Rochester/USA (May)
University of Strasbourg/France (June)
Bielefeld University/Germany (July)
Technical University of Braunschweig/Germany (September)