大洞 光司
大洞 光司Koji OOHORA
- 所属
大阪大学大学院 工学研究科 応用化学専攻 准教授
- 居室
吹田キャンパス 工学研究科C4棟 623号室
- 連絡先
Phone: 06-6879-7929
Fax: 06-6879-7930
E-mail: oohora “at” chem.eng.osaka-u.ac.jp (“at” = @)
経歴
1985年 大阪府大阪市生まれ
- 学歴
2003.3 大阪府立大手前高等学校 卒業
2007.3 大阪大学工学部応用自然科学科 卒業
2009.3 大阪大学大学院工学研究科応用化学専攻博士前期課程修了
2011.9 大阪大学大学院工学研究科応用化学専攻博士後期課程修了- 学位
博士（工学）大阪大学
"Artificial Protein Assemblies Formed by Specific Interprotein Interactions via Heme Derivatives"- 留学
2010.1–2010.7 バーゼル大学（スイス）T. R. Ward 研究室 留学
2013.8-2013.10 アーヘン工科大学（ドイツ）U. Schwaneberg研究室 留学- 主要職歴・研究歴
2009.4–2011.9 日本学術振興会特別研究員（DC1）
2011.10–2015.6 大阪大学大学院工学研究科応用化学専攻 助教
2015.6–2020.3 大阪大学大学院工学研究科グローバル若手研究者フロンティア研究拠点
助教（テニュアトラック教員）
2015.12- 2019.3 科学技術振興機構さきがけ研究員（革新的触媒の科学と創製）
2020.4-現職 大阪大学大学院工学研究科応用化学専攻 准教授
研究分野
- 専門分野
生物無機化学、生体機能関連化学、超分子化学、錯体化学、有機合成化学、触媒化学
- 所属学会
日本化学会、錯体化学会、生物無機化学会(SBIC)、ポルフィリン・フタロシアニン学会(SPP)
- 研究キーワード
ポルフィリン、ヘム、ヘムタンパク質、ヘム酵素、補欠分子族、コファクター、ミオグロビン、ヘモグロビン、シトクロムb562、アビジン、ストレプトアビジン、ビオチン、酸素錯体、生体金属、生体高分子、超分子、ナノマテリアル、機能材料、有機合成、錯体合成、タンパク質発現、タンパク質工学、遺伝子工学、反応解析、タンパク質機能改変、自己組織化、分子認識、原子間力顕微鏡、分子動力学計算
受賞暦
2008 The 4th Asian Biological Inorganic Chemistry Conference Young Scientist Oral Presentation Award 2011 15th International Conference on Biological Inorganic Chemistry SBIC Student Travel Grants 2013 第29回井上研究奨励賞 2013 日本化学会第93春季年会 優秀講演賞（学術） 2014 第8回バイオ関連化学シンポジウム 講演賞 2015 大阪大学総長奨励賞2015（研究部門） 2019 PCCP Prize 2019 2019 日本化学会第99春季年会 優秀講演賞（学術） 2019 Early Career Award in 9th International Symposium on Bioorganometallic Chemistry 2021 日本化学会 第70回進歩賞 2021 錯体化学会 研究奨励賞
研究業績
- 主要論文
1. Supramolecular Hemoprotein Linear Assembly by Successive interprotein Heme–Heme Pocket Interactions
Hiroaki Kitagishi, Koji Oohora, Hiroyasu Yamaguchi, Hideki Sato, Takashi Matsuo, Akira Harada, Takashi Hayashi*
J. Am. Chem. Soc., 129, 10326–10327 (2007).2. Self-Assembly of One- and Two-Dimensional Hemoprotein Systems by Polymerization through Heme–Heme Pocket Interactions
Hiroaki Kitagishi, Yasuaki Kakikura, Hiroyasu Yamaguchi, Koji Oohora, Akira Harada, Takashi Hayashi*
Angew. Chem., Int. Ed., 48, 1271–1274 (2009). Issue Picture3. Thermodynamically Controlled Supramolecular Polymerization of Cytochrome b562
Hiroaki Kitagishi, Koji Oohora, Takashi Hayashi*
Biopolymers, 3, 194–200 (2009). Front Cover4. A Chemically-controlled Supramolecular Protein Polymer Formed by a Myoglobin-based Self-assembly System
Koji Oohora, Akira Onoda, Hiroaki Kitagishi, Hiroyasu Yamaguchi, Akira Harada, Takashi Hayashi*
Chem. Sci., 2, 1033–1038, (2011). Backside Cover.5. Chemically Programmed Supramolecular Assembly of Hemoprotein and Streptavidin with Alternating Alignment
Koji Oohora, Sabina Burazerovic, Akira Onoda, Yvonne M. Wilson, Thomas R. Ward* Takashi Hayashi*
Angew. Chem., Int. Ed., 51, 3818–3821 (2012). Front Cover.6. Fibrous Supramolecular Assemblies Connected with Synthetic Heme Dimer and Apohemoprotein Dimer
Akira Onoda, Akinori Takahashi, Koji Oohora, Yoshitaka Onuma, Takashi Hayashi*
Chem. Biodivers., 9, 1684–1692 (2012).7. Supramolecular Assembling Systems Formed by Heme–Heme Pocket Interactions in Hemoproteins
Koji Oohora, Akira Onoda, Takashi Hayashi*
Chem. Commun., 48, 11714–11726 (2012). Feature Article.8. Photoinduced Electron Transfer of ZnS−AgInS2 Solid-Solution Semiconductor Nanoparticles: Emission Quenching and Photocatalytic Reactions Controlled by Electrostatic Forces
Taro Uematsu, Akihisa Doko, Tsukasa Torimoto, Koji Oohora, Takashi Hayashi, Susumu Kuwabata*
J. Phys. Chem. C, 117, 15667–15676 (2013).9. C(sp3)–H Bond Hydroxylation Catalyzed by Myoglobin Reconstituted with Manganese Porphycene
Koji Oohora, Yushi Kihira, Eiichi Mizohata, Tsuyoshi Inoue, Takashi Hayashi*
J. Am. Chem. Soc., 135, 17282–17285 (2013).10. “Hemoprotein-based Supramolecular Assembling Systems” Koji Oohora, and Takashi Hayashi* Curr. Opin. Chem. Biol., 19, 154–161 (2014).
11. “Co(II)/Co(I) Reduction-induced Axial Histidine-flipping in Myoglobin Reconstituted with a Cobalt Tetradehydrocorrin as a Methionine Synthase Model” Takashi Hayashi,* Yoshitsugu Morita, Eiichi Mizohata, Koji Oohora, Jun Ohbayashi, Tsuyoshi Inoue, and Yoshio Hisaeda Chem. Commun., 50, 12560–12563 (2014). Open Access.
12. “meso-Dibenzoporphycene has a Large Bathochromic Shift and a Porphycene Framework with an Unusual Cis Tautomeric Form” Koji Oohora, Ayumu Ogawa, Tamaki Fukuda, Akira Onoda, Jun-ya Hasegawa,* and Takashi Hayashi* Angew. Chem., Int. Ed., 54, 6227–6230 (2015).
13. “Energy Migration within Hexameric Hemoprotein Reconstituted with Zn Porphyrinoid Molecules” Koji Oohora, Tsuyoshi Mashima, Kei Ohkubo, Shunichi Fukuzumi, and Takashi Hayashi* Chem. Commun., 51, 1138–1140 (2015). Open Access.
14. “Intraprotein Transmethylation via a CH3–Co(III) Species in Myoglobin Reconstituted with a Cobalt Corrinoid Complex” Yoshitsugu Morita, Koji Oohora, Akiyoshi Sawada, Kazuki Doitomi, Jun Ohbayashi, Takashi Kamachi, Kazunari Yoshizawa, Yoshio Hisaeda, and Takashi Hayashi* Dalton Trans., 45, 3277–3284 (2016). Open Access. Front Cover.
15. “Crystal Structures and Coordination Behavior of Aqua- and Cyano-Co(III) Tetradehydrocorrins in the Heme Pocket of Myoglobin” Yoshitsugu Morita, Koji Oohora, Eiichi Mizohata, Akiyoshi Sawada, Takashi Kamachi, Kazunari Yoshizawa, Tsuyoshi Inoue, and Takashi Hayashi* Inorg. Chem., 55, 1287–1295 (2016).
16. “Oxygen-binding Protein Fiber and Microgel: Supramolecular Myoglobin-Poly(acrylate) Conjugates” Toshikazu Ono, Yasushi Hisaoka, Akira Onoda,* Koji Oohora, and Takashi Hayashi* Chem. Asian J., 11, 1036-1042 (2016).
17. “Redox Potentials of Cobalt Corrinoids with Axial Ligands Correlate with Heterolytic Co–C Bond Dissociation Energies” Yoshitsugu Morita, Koji Oohora, Akiyoshi Sawada, Takashi Kamachi, Kazunari Yoshizawa, and Takashi Hayashi* Inorg. Chem., 56, 1950–1955 (2017).
18. “Cobalt tetradehydrocorrins coordinated by imidazolate-like histidine in the heme pocket of horseradish peroxidase” Koji Oohora, Ning Tang, Yoshitsugu Morita, and Takashi Hayashi* J. Biol. Inorg. Chem., 22, 695–703 (2017) .
19. “Iron-Strapped Porphyrins with Carboxylic Acid Groups Hanging over the Coordination Site: Synthesis, X-ray Characterization, and Dioxygen Binding” Bernard Boitrel*, Ismail Hijazi, Thierry Roisnel, Koji Oohora, and Takashi Hayashi* Inorg. Chem., 56, 7373–7383 (2017).
20. “A supramolecular assembly based on an engineered hemoprotein exhibiting a thermal stimulus-driven conversion to a new distinct supramolecular structure” Koji Oohora, Yoshitaka Onuma, Yuta Tanaka, Akira Onoda, and Takashi Hayashi* Chem. Commun., 53, 6879–6882 (2017).
21. “CuAAC in a Distal Pocket: Metal Active-Template Synthesis of Strapped-Porphyrin [2]Rotaxanes” Yuta Miyazaki, Christophe Kahlfuss, Ayumu Ogawa, Takashi Matsumoto, Jennifer A. Wytko, Koji Oohora, Takashi Hayashi,* and Jean Weiss* Chem. Eur. J., 23, 13579–13582 (2017), Front Cover.
22. “Catalytic Cyclopropanation by Myoglobin Reconstituted with Iron Porphycene: Acceleration of Catalysis due to Rapid Formation of the Carbene Species” Koji Oohora, Hiroyuki Meichin, Liming Zhao, Matthew W, Wolf, Akira Nakayama, Jun-ya Hasegawa,* Nicolai Lehnert* and Takashi Hayashi* J. Am. Chem. Soc., 139, 17265–17268 (2017).
23. “Manganese(V) Porphycene Complex Responsible for Inert C–H Bond Hydroxylation in a Myoglobin Matrix” Koji Oohora*, Hiroyuki Meichin, Yushi Kihira, Hiroshi Sugimoto, Yoshitsugu Shiro, and Takashi Hayashi* J. Am. Chem. Soc.,139, 18460–18463 (2017).
24. “Substitution of an amino acid residue axially coordinating to the heme molecule in hexameric tyrosine-coordinated hemoprotein to enhance peroxidase activity” Tsuyoshi Mashima, Koji Oohora,* and Takashi Hayashi* J. Porphyrins Phthalocyanines, 21, 824–831 (2017).
25. “Substitution of an amino acid residue axially coordinating to the heme molecule in hexameric tyrosine-coordinated hemoprotein to enhance peroxidase activity” Shumpei Hanai, Hirofumi Tsujino, Taku Yamashita,* Ryo Torii, Hitomi Sawai, Yoshitsugu Shiro, Koji Oohora, Takashi Hayashi and Tadayuki Uno* J. Inorg. Biochem.,179, 1–9 (2018).
26. “Successive Energy Transfer within Multiple Photosensitizers Assembled in a Hexameric Hemoprotein Scaffold” Tsuyoshi Mashima, Koji Oohora* and Takashi Hayashi* Phys. Chem. Chem. Phys., 20, 3200–3209 (2018).
27. “Preparation and characterization of myoglobin reconstituted with Fe(II) oxaporphyrin: The monoanionic macrocycle provides unique cyanide binding behavior for the ferrous species” Hiroyuki, Meichin, Koji Oohora, and Takashi Hayashi* Inorg. Chim. Acta, 472, 184–191 (2018).
28. “Supramolecular Hemoprotein Assembly with a Periodic Structure Showing Heme–Heme Exciton Coupling” Koji Oohora,* Nishiki Fujimaki, Ryota Kajihara, Hiroki Watanabe, Takayuki Uchihashi,* and Takashi Hayashi* J. Am. Chem. Soc., 140, 10145–10148 (2018).
29. “Synthesis and Characterization of meso-Substituted Cobalt Tetradehydrocorrin and Evaluation of Its Electrocatalytic Behavior Toward CO2 Reduction and H2 Evolution” Ayumu Ogawa, Koji Oohora* and Takashi Hayashi* Inorg. Chem., 57, 14644–14652 (2018).
30. “Electrochemical CO2 reduction by a cobalt bipyricorrole complex: decrease of an overpotential value derived from monoanionic ligand character of the porphyrinoid species” Ayumu Ogawa, Koji Oohora,* Wenting Gu and Takashi Hayashi* Chem. Commun., 55, 493–496 (2019).
31. “A ring-shaped hemoprotein trimer thermodynamically controlled by the supramolecular heme–heme pocket interaction” Koji Oohora,* Ryota Kajihara, Nishiki Fujimaki, Takayuki Uchihashi* and Takashi Hayashi* Chem. Commun., 55, 1544–1547 (2019), Front Cover.
32. “Photoinduced electron transfer within supramolecular hemoprotein co-assemblies and heterodimers containing Fe and Zn porphyrins” Ryota Kajihara, Koji Oohora,* and Takashi Hayashi* J. Inorg. Biochem., 193, 42–51 (2019).
33. “Arginine Residues Provide a Multivalent Effect for Cellular Uptake of a Hemoprotein Assembly” Koji Oohora,* Ryota Kajihara, Misa Jiromaru, Hiroaki Kitagishi* and Takashi Hayashi* Chem. Lett., 48, 295–298 (2019).
34. “Light Triggers Molecular Shuttling in Rotaxanes: Control over Proximity and Charge Recombination” Maximilian Wolf,a Ayumu Ogawa, Mareike Bechtold, Maxime Vonesch, Jennifer A. Wytko, Koji Oohora, Stéphane Campidelli, Takashi Hayashi,* Dirk M. Guldi,* and Jean Weiss* Chem. Sci., 10, 3846–3853 (2019).
35. “Hemoproteins Reconstituted with Artificial Metal Complexes as Biohybrid Catalysts” Koji Oohora, Akira Onoda and Takashi Hayashi* Acc. Chem. Res., 52, 945–954 (2019).
36. “Myoglobin Reconstituted with Ni Tetradehydrocorrin as a Methane Generating Model of Methyl‐coenzyme M Reductase” Koji Oohora,* Yuta Miyazaki and Takashi Hayashi* Angew. Chem. Int. Ed., 58, 13813–13817 (2019)
37. “Supramolecular dimerization of a hexameric hemoprotein via multiple pyrene–pyrene interactions” Koji Oohora,* Shota Hirayama, Tsuyoshi Mashima and Takashi Hayashi* J. Porphyrins Phthalocyanines, 24, 259–267 (2020).
38. “Methane generation via intraprotein C–S bond cleavage in cytochrome b562 reconstituted with nickel didehydrocorrin” Yuta Miyazaki, Koji Oohora* and Takashi Hayashi* J. Organomet. Chem., 901, 120945 (2020).
39. “Construction of a Hexameric Hemoprotein Sheet and Direct Observation of Dynamic Process of its Formation” Koji Oohora,* Shota Hirayama, Takayuki Uchihashi* and Takashi Hayashi* Chem. Lett., 49, 186-190 (2020).
40. “A Thermoresponsive Micellar Assembly Constructed from a Hexameric Hemoprotein Modified with Poly(N-isopropylacrylamide) toward an Artificial Light-harvesting System” Shota Hirayama, Koji Oohora,* Takayuki Uchiahashi* and Takashi Hayashi* J. Am. Chem. Soc., 142. 1822–1831 (2020).
41. "Methane Generation and Reductive Debromination of Benzylic Position by Reconstituted Myoglobin Containing Nickel Tetradehydrocorrin as a Model of Methyl-coenzyme M Reductase” Yuta Miyazaki, Koji Oohora,* and Takashi Hayashi* Inorg. Chem., 59, 11995-12004 (2020). 42. “Effect of Molecule–Substrate Interactions on the Adsorption of meso-Dibenzoporphycene Tautomers Studied by Scanning Probe Microscopy and First-Principles Calculations” Tomoko K. Shimizu, Carlos Romero-Muñiz, Oleksandr Stetsovych, Jaime Carracedo-Cosme, Michael Ellner, Pablo Pou, Koji Oohora, Takashi Hayashi, Ruben Perez* and Oscar Custance* J. Phys. Chem. C, 124, 26759-26768 (2020). 43. "Myoglobins engineered with artificial cofactors serve as artificial metalloenzymes and models of natural enzymes” Koji Oohora* and Takashi Hayashi* Dalton Trans., 50, 1940-1949 (2021) Front Cover. 44. “A Supramolecular Assembly of Hemoproteins Formed in a Star-Shaped Structure via Heme–Heme Pocket Interactions” Julian Wong Soon, Koji Oohora,* Shota Hirayama, and Takashi Hayashi* Int. J. Mol. Sci., 22, 1012 (2021) Open Access.
45. “Dynamic Protease Activation on a Multimeric Synthetic Protein Scaffold via Adaptable DNA-Based Recruitment Domains” Tsuyoshi Mashima, Bas J. H. M. Rosier, Koji Oohora, Tom F. A. de Greef, Takashi Hayashi, and Luc Brunsveld*Angew. Chem. Int. Ed., 60, 11262–11266 (2021). Open Access. 46. "Functional Myoglobin Model Composed of a Strapped Porphyrin/Cyclodextrin Supramolecular Complex with an Overhanging COOH That Increases O2/CO Binding Selectivity in Aqueous Solution"Qiyue Mao, Pradip K. Das, Stéphane Le Gac, Bernard Boitrel,* Vincent Dorcet, Koji Oohora, Takashi Hayashi, and Hiroaki Kitagishi*Inorg. Chem., 60, 12392–12404 (2021). - 著書
1. Koji Oohora, Takashi Hayashi, “Incorporation of modified and artificial cofactors into naturally occurring protein scaffolds” In Methods in Molecular Biology, pp. 251–263,2014.
2.Koji Oohora, Takashi Hayashi "Artificial Hemoprotein Assemblies in Development of Nanobiomaterials" In Nioinspired Chemistry From Enzymes to Synthetic Models, M. Réglier, Ed., World Scientific, 71–88 (2019).
3. Koji Oohora, Takashi Hayashi "Myoglobin Derivatives Reconstituted with Modified Metal Porphyrinoids, as a Structural and Functional Models of the Cytochrome P450 Enzymes" In Dioxygene-dependent Heme Enzymes, M. Ikeda-Saito, E. Raven, Eds., RSC, 63–78 (2019).
- 招待講演
1. Koji Oohora, “C–H Bond Hydroxylation Catalyzed by Manganese Porphycene in a Myoglobin Matrix”
Characterization and Reactivity of Metal-oxo Species Generated by a Metalloenzyme Model, S4-4, Sep. 2014, Chuo University, Tokyo2. 大洞 光司 "ヘムタンパク質を基盤とした超分子集合体および人工酵素の調製" 第28回生物無機化学夏季セミナー （山喜旅館、静岡）, 2015年8月28日 招待講演
3. Koji Oohora "Design and engineering of myoglobin containing a manganese porphycene toward enantioselective hydroxylase" 第53回日本生物物理学会年会 （金沢大学、石川）, 2015年9月14日 招待講演
4. Koji Oohora, Yasunobu Tanaka, Ayumu Ogawa, Takashi Hayashi "Synthesis, structure and physicochemical properties of meso-dibenzoporphycene" Theory, Measurement and Creation of Porphyrinoid Compounds as Soft Molecular Systems (物質・材料研究機構、茨城)、2015年10月1日 招待講演
5. Koji Oohora, Takashi Hayashi "H2O2-dependent hydroxylation of C–H bond by myoglobin reconstituted with a manganese porphycene complex" PACIFICHEM 2015 （ヒルトンワイアンビレッジ、ハワイ）、2015年12月17日、招待講演
6. Koji Oohora, Ayumu Ogawa, Yasunobu Tanaka, Jun-ya Hasegawa, Takashi Hayashi, “Dibenzoporphycene with Annulated Benzene Rings at Two Ethylene Bridges of the Porphycene Framework” Ninth International Conference on Porphyrins and Phthalocyanines （Zhongshan Hotel, 南京）、2016年7月7日、招待講演
7. 大洞 光司、"ヘムタンパク質の機能操作：超分子集合体および人工酵素の構築" 機能物性セミナー（東京大学物性研究所、千葉）, 2016年7月21日、招待講演
8.Koji Oohora "Hemoprotein engineered with unnatural cofactors toward artificial metalloenzymes" 日本化学会第97春季年会（Asian International Symposium -Coordination Chemistry, Organometallic Chemistry-）、(慶応大学、神奈川) 2017年3月17日 招待講演
9. 大洞 光司 "ヘムタンパク質集合体を基盤とする人工光捕集系の構築" 第38回光化学若手の会（休暇村志賀島、福岡）2017年6月16日、招待講演
10. 大洞 光司"生体材料および人工酵素を指向したヘムタンパク質の化学的改変" 錯体化学若手の会夏の学校2017（ホテルたつき、愛知）2017年7月31日、招待講演
11. 大洞 光司 "ヘムタンパク質の化学的改変による人工酵素・酵素モデルの開発" ITbM/IGER Chemistry Workshop 2017（名古屋大学、愛知）2017年11月6日、招待講演
12. Koji Oohora, Takashi Hayashi "An Array of Metal Porphyrinoid Chromophores Using Hemoprotein Assembly as a Scaffold" SNCPP18, （立命館大学、滋賀）2018年6月16日、招待講演
13. Koji Oohora "Construction and photochemical properties of supramolecular assembly of hemoprotein" SUPRAMOLECULAR CHEMISTRY OF NITROGEN LIGANDS （Univ. Strasbourg, France） 2018年6月29日、招待講演
14. Koji Oohora, Tang Ning, Yoshitsugu Morita, Takashi Hayashi, "Hemoproteins reconstituted with synthetic cobalt corrinoid toward models of vitamin B12-dependent enzymes" Tenth International Conference on Porphyrins and Phthalocyanines, (Westin Grand in Munich, Germany) 2018年7月3日、招待講演
15. Koji Oohora,“Hemoproteins reconstituted with metal corrinoids as functional models of natural enzymes related to bioorganometallic species” 9th International Symposium on Bioorganometallic Chemistry (Univ. York, United Kingdom) 2019年7月31日、基調講演（Keynote lecture）
- Copyright(c) 2015- Hayashi Lab, Osaka Univ. All Rights Reserved.
